β-Gal Assay Kit
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Invitrogen™

β-Gal Assay Kit

The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected자세히 알아보기
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카탈로그 번호수량
K14550180 mL
카탈로그 번호 K145501
제품 가격(KRW)
1,146,000
Online offer
Ends: 31-Dec-2025
1,348,000
할인액 202,000 (15%)
Each
카트에 추가하기
수량:
80 mL
제품 가격(KRW)
1,146,000
Online offer
Ends: 31-Dec-2025
1,348,000
할인액 202,000 (15%)
Each
카트에 추가하기
The β-Gal Assay Kit provides the reagents required to quickly measure the levels of active β-galactosidase expressed in cells transfected with plasmids expressing the lacZ gene. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of β-galactosidase such as ortho-nitrophenyl-D-galactopyranoside (ONPG). Hydrolysis of ONPG to the ONP anion produces a bright yellow color with a peak absorbance at 420 nm that can be quantified using a spectrophotometer.
10.7 gene expression
For Research Use Only. Not for use in diagnostic procedures.
사양
어세이β-Gal (lacZ) Assay
제품 유형Beta-Gal Assay Kit
수량80 mL
기질ONPG
타겟Beta-Galactosidase
검출 방법Colorimetric
Unit SizeEach
구성 및 보관
The β-Gal Assay Kit contains 10X PBS (phosphate buffered saline), lysis buffer, cleavage buffer, β-mercaptoethanol, ONPG, stop buffer. Store ONPG at -20°C. All other components are stored at either room temperature or +4°C. All reagents are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

Are detergents compatible with the β-Gal (ONPG) assay kit (K1455-01)?

Neither NP40 nor Triton X-100 (1%) should interfere with the reaction, although there is a chance that a precipitate may form when the detergent is added to the b-Gal assay reagent.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.