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Invitrogen™
PureLink™ Total RNA Blood Kit
PureLink™ Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 μl fresh, whole mammalian blood,자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K156001 | 50 preps |
카탈로그 번호 K156001
제품 가격(KRW)
538,000
Online offer
Ends: 31-Dec-2025
597,000할인액 59,000 (10%)
Each
수량:
50 preps
제품 가격(KRW)
538,000
Online offer
Ends: 31-Dec-2025
597,000할인액 59,000 (10%)
Each
PureLink™ Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 μl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes
Simple RNA Purification from Whole Blood
PureLink™ Total RNA Blood Kit is designed for purifying from 50 to 500 μl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.
Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink™ Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink™ Total RNA Blood Kit.
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytes
Simple RNA Purification from Whole Blood
PureLink™ Total RNA Blood Kit is designed for purifying from 50 to 500 μl fresh whole blood or approximately 3.5 x 106 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.
Leukocyte Enrichment for Improved RNA Purification
Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink™ Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink™ Total RNA Blood Kit.
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량30–100 μL
최종 제품 유형Total RNA
용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
고처리량 호환성Not High-throughput Compatible (Manual)
정제 시간20 min. (After sample homogenization)
수량50 preps
스케일Mini
배송 조건Room Temperature
시작 물질 양Blood: ≤500 μL
수율1000 μg (Binding capacity)
Isolation TechnologySilica Spin Column
샘플 종류Blood
Unit SizeEach
구성 및 보관
• 17.5 mL Lysis Buffer L3; store at room temperature
• 175 mL Lysis Buffer L5; room temperature
• 60 mL Wash Buffer W4; room temperature
• 10 mL Wash Buffer W5; room temperature
• 5 mL RNase-free Water; room temperature
• 50 Spin Cartridges with Collection Tubes; room temperature
• 50 Wash Tubes (2 mL); room temperature
• 50 Elution Tubes (1.7 mL); room temperature
• 175 mL Lysis Buffer L5; room temperature
• 60 mL Wash Buffer W4; room temperature
• 10 mL Wash Buffer W5; room temperature
• 5 mL RNase-free Water; room temperature
• 50 Spin Cartridges with Collection Tubes; room temperature
• 50 Wash Tubes (2 mL); room temperature
• 50 Elution Tubes (1.7 mL); room temperature