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Invitrogen™
mRNA Catcher™ PLUS Purification Kit
The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.
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보기 방식 변경
| 카탈로그 번호 | 수량 | 제품 유형 |
|---|---|---|
| K157002 | 96 Preps | Purification Kit |
| K157003 | 960 Preps | Purification Kit |
| K157004 K1570-04으로도 사용됨 | 960 Preps | Buffers Only |
카탈로그 번호 K157002
제품 가격(KRW)
602,000
Each
수량:
96 Preps
제품 유형:
Purification Kit
제품 가격(KRW)
602,000
Each
The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of locked nucleic acid (LNA) technology, the mRNA Catcher PLUS kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.
The mRNA Catcher PLUS Purification Kit offers:
- Robust performance—high binding capacity per well (>80 ng/well)
- Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
- Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
- Compatible plate design—may be used with most PCR cyclers
How it works
mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamLined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량80 μL
최종 제품 유형mRNA
용도(애플리케이션)RT-PCR, qPCR, cDNA Library Construction, Microarray Analysis
용도(장비)Robotic Liquid Handling Systems
고처리량 호환성High-throughput Compatible
제품 유형Purification Kit
정제 시간1.5 hr. (Including sample homogenization)
수량96 Preps
배송 조건Room Temperature
시작 물질 양Cells: 100–106, Tissue: 4–40 mg, Total RNA: 0.1–100 μg, Whole Blood: 40 μL
수율>80 ng
Isolation TechnologyOligo dT Affinity
샘플 종류Cells, Tissue, Total RNA, Whole Blood
Unit SizeEach
구성 및 보관
• 1 x 96-well mRNA Catcher™ PLUS Plate; store at 4°C
• 4 mL 2X Lysis Buffer L20; room temperature
• 30 mL Wash Buffer W15; room temperature
• 4 mL Dilution Buffer DL1; room temperature
• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature
• 100 μL 0.5M DTT; 4°C
• 4 mL 2X Lysis Buffer L20; room temperature
• 30 mL Wash Buffer W15; room temperature
• 4 mL Dilution Buffer DL1; room temperature
• 8 mL Elution Buffer E3 (DEPC-treated RNase-free water); room temperature
• 100 μL 0.5M DTT; 4°C