mRNA Catcher™ PLUS Purification Kit
mRNA Catcher™ PLUS Purification Kit
Actual product may vary
Invitrogen™

mRNA Catcher™ PLUS Purification Kit

The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format.
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카탈로그 번호수량제품 유형
K157004
K1570-04으로도 사용됨
960 PrepsBuffers Only
K15700296 PrepsPurification Kit
K157003960 PrepsPurification Kit
카탈로그 번호 K157004
K1570-04으로도 사용됨
제품 가격(KRW)
-
수량:
960 Preps
제품 유형:
Buffers Only
The mRNA Catcher PLUS Purification Kit enables high-throughput purification of mRNA from cells, tissues, blood, and total RNA in a 96-well format. By taking advantage of locked nucleic acid (LNA) technology, the mRNA Catcher PLUS kit provides improved performance and a faster protocol, while preserving mRNA yield and purity.

The mRNA Catcher PLUS Purification Kit offers:

  • Robust performance—high binding capacity per well (>80 ng/well)
  • Reproducible results—low CVs from well-to-well and plate-to-plate (∼2%)
  • Optimized protocols—for small sample sizes (100 cells, 4 mg tissue, and 40 μL blood)
  • Compatible plate design—may be used with most PCR cyclers

How it works

mRNA from total RNA, cells, tissue, and blood samples is hybridized to an immobilized, single-stranded 20-mer oligo(dT)-containing LNA. The incorporation of LNA into the oligo probe increases the hybridization efficiency and specificity of the mRNA binding. The mRNA can either be eluted or reversed-transcribed to cDNA directly in the well. The mRNA Catcher PLUS method does not require centrifugation, precipitation, phase separation, or column filtration. In addition, the entire method is amenable to automation for streamLined, high-throughput expression profiling for large-scale processes. Isolated mRNA is suitable for use in downstream applications such as RT-PCR and qRT-PCR.

For Research Use Only. Not for use in diagnostic procedures.
사양
반응 수960 reactions
제품 유형Buffers Only
수량960 Preps
배송 조건Room Temperature
Unit SizeEach
구성 및 보관
Store at room temperature.

자주 묻는 질문(FAQ)

What types of samples can be used to isolate mRNA with the mRNA Catcher PLUS?

The mRNA Catcher PLUS kit is designed to isolate mRNA from cells, animal tissues, whole blood, and total RNA.

How many cells should I use for RNA isolation with the mRNA CatcherPLUS Kit?

We recommend using a minimum of 100 cells per well for mRNA isolation with the mRNA CatcherPLUS Kit.

Which PCR machines is the plate in mRNA CatcherPLUS Kit compatible with?

The mRNA CatcherPLUS Kit plate should fit in any standard 96-well PCR machine including MJ and ABI thermocyclers.