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인용 및 참조 문헌 (20)
Invitrogen™
Easy-DNA™ gDNA Purification Kit
The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K180001 | 1 Kit |
카탈로그 번호 K180001
제품 가격(KRW)
848,000
Online offer
Ends: 31-Mar-2026
969,000할인액 121,000 (12%)
Each
수량:
1 Kit
제품 가격(KRW)
848,000
Online offer
Ends: 31-Mar-2026
969,000할인액 121,000 (12%)
Each
The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:
• Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
• Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
• Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types
Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
• Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
• Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
• Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types
Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
For Research Use Only. Not for use in diagnostic procedures.
사양
용리량10 to 150 μL
최종 제품 유형gDNA
용도(애플리케이션)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
고처리량 호환성Not High-throughput Compatible (Manual)
수량1 Kit
샘플 종류Bacteria, Cells, Hair Follicles, Mouse Tails, Plant, Tissue, Virus, Whole Blood, Yeast
배송 조건Room Temperature
시작 물질 양Bacteria: ≤109 cells
Cells: ≤108
Hair Follicles: ≤5
Mouse Tails: 1 cm
Plant: ≤50 mg
Tissue: ≤1 g
Virus: ≤750 μL
Whole Blood: ≤2 mL
Yeast: ≤10 mL
Cells: ≤108
Hair Follicles: ≤5
Mouse Tails: 1 cm
Plant: ≤50 mg
Tissue: ≤1 g
Virus: ≤750 μL
Whole Blood: ≤2 mL
Yeast: ≤10 mL
테스트 시간Up to 90 min.
수율≤850 μg
Isolation TechnologyOrganic Extraction
Unit SizeEach
구성 및 보관
• 55 mL Solution A; room temperature
• 25 mL Solution B; room temperature
• 100 mL TE Buffer; room temperature
• 750 μL Mussel Glycogen; room temperature, or -20°C for long-term storage
• 750 μL RNase; room temperature, or -20°C for long-term storage
• 750 μL Protein Degrader; room temperature, or -20°C for long-term storage
• 25 mL Solution B; room temperature
• 100 mL TE Buffer; room temperature
• 750 μL Mussel Glycogen; room temperature, or -20°C for long-term storage
• 750 μL RNase; room temperature, or -20°C for long-term storage
• 750 μL Protein Degrader; room temperature, or -20°C for long-term storage
자주 묻는 질문(FAQ)
What type of samples can be used for the isolation of DNA using the Easy-DNA Kit?
인용 및 참조 문헌 (20)
인용 및 참조 문헌
Abstract
Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection.
Journal:Silence
PubMed ID:23273270
'High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA;
Participation of fad and mbt genes in synthesis of mycobactin in Mycobacterium smegmatis.
Journal:J Bacteriol
PubMed ID:14702306
'Colonies of Mycobacterium smegmatis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the accumulation in the cells of the deferri form of the siderophore mycobactin. Two mutants with little or no fluorescence, designated LUN8 and LUN9, were isolated by screening colonies of
Resistance to murine AIDS in offspring of mice infected with LP-BM5. Role of CD8 T cells.
Journal:J Immunol
PubMed ID:8648122
'The murine-acquired immunodeficiency syndrome (MAIDS) is caused by a mixture of murine leukemia viruses (LP-BM5 MuLV). The influence of perinatal contact with retroviruses or their Ags on the response to infection was tested by infecting with LP-BM5 (MuLV) the adult offspring of mice with MAIDS. These offspring were resistant to
Inducible expression of a dominant negative DNA polymerase-gamma depletes mitochondrial DNA and produces a rho0 phenotype.
Journal:J Biol Chem
PubMed ID:12645575
'We report the inducible, stable expression of a dominant negative form of mitochondria-specific DNA polymerase-gamma to eliminate mitochondrial DNA (mtDNA) from human cells in culture. HEK293 cells were transfected with a plasmid encoding inactive DNA polymerase-gamma harboring a D1135A substitution (POLGdn). The cells rapidly lost mtDNA (t1/2 = 2-3 days)
Characterization and sequencing of prototypic human T-lymphotropic virus type 1 (HTLV-1) from and HTLV-1/2 seroindeterminate patient.
Journal:J Virol
PubMed ID:10666247
'Serological screening for human T-lymphotropic virus type 1 (HTLV-1) parallels the standard screening process for human immunodeficiency virus (HIV), in which samples found positive by enzyme-linked immunosorbent assay (ELISA) are confirmed with a modified Western blot procedure. There are a significant number of cases in which HTLV-1/2 ELISA-positive specimens demonstrate