Tango™ GPR44-bla U2OS cells contain the human G protein coupled receptor 44 (GPR44) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase reporter gene under the control of a UAS response element. The Tango™ GPR44-bla U2OS cells have been functionally validated for Z’ factor and EC50 concentrations of Indomethacin. In addition, Tango™ GPR44-bla U2OS cells have been tested for assay performance under variable conditions.
For Research Use Only. Not for use in diagnostic procedures.
사양
등록 번호
NM_004778
어세이 입력
Cell-based beta-arrestin recruitment
셀 상태
Dividing Cells
세포질 후미
Tail from GRPR GPCR
검출 방법
Fluorescent
용도(애플리케이션)
FRET
인간 단백질 분류
G-Protein Coupled Receptor (GPCR)
개체군 상태
Single Cell Clone Origin
제품라인
Tango™
판독
End Point
타겟 입력
CRTH2, GPR44
세포주
U2OS
System Type
Tango™
Druggable Target
GPCR
구성 및 보관
Tango™ GPR44 (CRTH2)-bla U2OS cells are shipped on dry ice, 8 x 10^6 cells⁄vial. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.