S.N.A.P.™ Plasmid DNA MidiPrep Kit
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Invitrogen™

S.N.A.P.™ Plasmid DNA MidiPrep Kit

The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid자세히 알아보기
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카탈로그 번호수량
K19100120 preps
카탈로그 번호 K191001
제품 가격(KRW)
947,000
Online offer
Ends: 31-Mar-2026
1,082,000
할인액 135,000 (12%)
Each
카트에 추가하기
수량:
20 preps
제품 가격(KRW)
947,000
Online offer
Ends: 31-Mar-2026
1,082,000
할인액 135,000 (12%)
Each
카트에 추가하기
The S.N.A.P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.N.A.P.™ MidiPrep procedure takes just 40 minutes. You simply:

• Lyse cells and filter debris with the S.N.A.P.™ filtering column
• Apply flow-through to the S.N.A.P.™ DNA binding column
• Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE buffer

Plasmid DNA purified with the S.N.A.P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형Plasmid DNA
용도(애플리케이션)Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
고처리량 호환성Not High-throughput Compatible (Manual)
프렙 스케일100-200 μg (Medium-Scale) Plasmid DNA
제품라인S.N.A.P.
제품 유형Plasmid DNA MidiPrep Kit
수량20 preps
샘플 종류Bacterial Culture
스케일Midi
타겟Plasmid DNA
테스트 시간40 min.
형식Kit
Isolation TechnologySpin Column
Unit SizeEach
구성 및 보관
Store the S.N.A.P.™ MidiPrep filtering columns, S.N.A.P.™ MidiPrep DNA binding columns, lysis buffer, binding buffer, wash buffer, and 4X final wash at room temperature. Store the resuspension buffer, precipitation salt, and RNase A at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

Which centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol?

The Sorval RT6000B table-top centrifuge is used at Thermo Fisher Scientific for the S.N.A.P. MidiPrep protocol. The low-speed spins are around 2,000 rpm and the high-speed spins are around 3,000 rpm. You may also use the Sorval RC-5B centrifuge with the SS-34 rotor. However, you might observe some spillage with this centrifuge.

What is the upper size limit for plasmid purification with the S.N.A.P. Miniprep Kit?

Bacmid DNA (>100 kbp) has been purified with the S.N.A.P. miniprep kit.

The PureLink HiPure Plasmid DNA Purification Kits (mini/midi/maxi scales) can also be used for large constructs. The HiPure kit manuals contain a modified protocol designed to help increase yields with these large plasmid isolations.

What are the compositions of the S.N.A.P. MiniPrep and MidiPrep Kit solutions?

The formulations are published in the manual for the S.N.A.P. MidiPrep kit, and are the same as the ones used in the MiniPrep kit.

What is the resin in the S.N.A.P. kits?

The column contains a silica/glass fiber based resin. However, the exact composition is proprietary.

Why is LB medium recommended for growing up plasmid preps in the S.N.A.P. protocol?

The volumes of culture recommended in our S.N.A.P. kits assume an OD of no more than 2.0. If a rich medium is used, the volume processed should be reduced to avoid overloading the column.