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인용 및 참조 문헌 (66)
Invitrogen™
TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli
TA Cloning™ Kit는 하룻밤이 걸리는 ligation 단계를 사용해 PRC 반응에서 직접 Taq-amplified PCR 산물을 클로닝하도록 만들어졌습니다. 이 kit는 pCR™ vector를자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 반응 수 | 벡터 |
|---|---|---|
| K200001 | 20 Reactions | pCRII |
| K200040 | 40 Reactions | pCR2.1 |
카탈로그 번호 K200001
제품 가격(KRW)
802,000
Online offer
Ends: 31-Dec-2025
943,000할인액 141,000 (15%)
Each
반응 수:
20 Reactions
벡터:
pCRII
제품 가격(KRW)
802,000
Online offer
Ends: 31-Dec-2025
943,000할인액 141,000 (15%)
Each
TA Cloning™ Kit는 하룻밤이 걸리는 ligation 단계를 사용해 PRC 반응에서 직접 Taq-amplified PCR 산물을 클로닝하도록 만들어졌습니다. 이 kit는 pCR™ vector를 사용하며 ≥90% 재조합체 함유 삽입물로 ≥80% 재조합체를 회수합니다. pCR™ vector의 일부 편리한 기능:
- 3'-T overhang으로 Taq-amplified PCR 산물의 직접 ligation
- 선택한 T7 (pCR™2.1) 또는 T7 및 Sp6 (pCR™II) promoter로 in vitro RNA transcription 및 sequencing 실시
- flanking EcoR I site의 다용도 polylinker로 삽입물 절개 용이
- kanamycin 또는 ampicillin 선택
- 쉬운 blue/white colony 스크리닝
- sequencing을 위한 M13 forward/reverse primer sites
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주INVαF ́
세포 유형Chemically Competent E. coli
클로닝 방법TA Cloning
용도(애플리케이션)PCR Cloning
반응 수20 Reactions
제품라인One Shot
제품 유형Cloning Kit
프로모터T7
수량20 reactions
벡터pCRII
형식Kit
Unit SizeEach
구성 및 보관
TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
인용 및 참조 문헌 (66)
인용 및 참조 문헌
Abstract
Hyperornithinaemia-hyperammonaemia-homocitrullinuria syndrome is caused by mutations in a gene encoding a mitochondrial ornithine transporter.
Journal:Nature genetics
PubMed ID:10369256
Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans.
Journal:Molecular and cellular biology
PubMed ID:10330150
Cryptococcus neoformans is a fungal pathogen that causes meningitis in patients immunocompromised by AIDS, chemotherapy, organ transplantation, or high-dose steroids. Current antifungal drug therapies are limited and suffer from toxic side effects and drug resistance. Here, we defined the targets and mechanisms of antifungal action of the immunosuppressant rapamycin in
Procollagen with skipping of alpha 1(I) exon 41 has lower binding affinity for alpha 1(I) C-telopeptide, impaired in vitro fibrillogenesis, and altered fibril morphology.
Journal:The Journal of biological chemistry
PubMed ID:11706004
Previous in vitro data on type I collagen self-assembly into fibrils suggested that the amino acid 776-796 region of the alpha1(I) chain is crucial for fibril formation because it serves as the recognition site for the telopeptide of a docking collagen monomer. We used a natural collagen mutation with a
Molecular characterization of Ancylostoma inhibitors of coagulation factor Xa. Hookworm anticoagulant activity in vitro predicts parasite bloodfeeding in vivo.
Journal:J Biol Chem
PubMed ID:11741914
'Bloodfeeding hookworms, which currently infect over a billion people in the developing world, are a leading cause of gastrointestinal hemorrhage and iron deficiency anemia. The major anticoagulant inhibitor of coagulation factor Xa has been identified from the hookworm parasite Ancylostoma ceylanicum using reverse transcription PCR and 3''-rapid amplification of cDNA
The catalytic mechanism of Cdc25A phosphatase.
Journal:J Biol Chem
PubMed ID:11805096
'Cdc25 phosphatases are dual specificity phosphatases that dephosphorylate and activate cyclin-dependent kinases (CDKs), thereby effecting the progression from one phase of the cell cycle to the next. Despite its central role in the cell cycle, relatively little is known about the catalytic mechanism of Cdc25. In order to provide insights