Search
인용 및 참조 문헌 (5)
Invitrogen™
TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli
TA Cloning™ Kit는 하룻밤이 걸리는 ligation 단계를 사용해 PRC 반응에서 직접 Taq-amplified PCR 산물을 클로닝하도록 만들어졌습니다. 이 kit는 pCR™ vector를자세히 알아보기
Have Questions?
보기 방식 변경
| 카탈로그 번호 | 반응 수 | 벡터 |
|---|---|---|
| K200040 | 40 Reactions | pCR2.1 |
| K200001 | 20 Reactions | pCRII |
카탈로그 번호 K200040
제품 가격(KRW)
1,720,000
Each
반응 수:
40 Reactions
벡터:
pCR2.1
제품 가격(KRW)
1,720,000
Each
TA Cloning™ Kit는 하룻밤이 걸리는 ligation 단계를 사용해 PRC 반응에서 직접 Taq-amplified PCR 산물을 클로닝하도록 만들어졌습니다. 이 kit는 pCR™ vector를 사용하며 ≥90% 재조합체 함유 삽입물로 ≥80% 재조합체를 회수합니다. pCR™ vector의 일부 편리한 기능:
- 3'-T overhang으로 Taq-amplified PCR 산물의 직접 ligation
- 선택한 T7 (pCR™2.1) 또는 T7 및 Sp6 (pCR™II) promoter로 in vitro RNA transcription 및 sequencing 실시
- flanking EcoR I site의 다용도 polylinker로 삽입물 절개 용이
- kanamycin 또는 ampicillin 선택
- 쉬운 blue/white colony 스크리닝
- sequencing을 위한 M13 forward/reverse primer sites
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주INVαF ́
세포 유형Chemically Competent E. coli
클로닝 방법TA Cloning
용도(애플리케이션)PCR Cloning
반응 수40 Reactions
제품라인One Shot
제품 유형Cloning Kit
프로모터T7
수량40 reactions
벡터pCR2.1
형식Kit
Unit SizeEach
구성 및 보관
TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid.
Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
인용 및 참조 문헌 (5)
인용 및 참조 문헌
Abstract
Genetic and epigenetic incompatibilities underlie hybrid dysgenesis in Peromyscus.
Journal:Nature Genetics
PubMed ID:10802670
'Crosses between the two North American rodent species Peromyscus polionotus (PO) and Peromyscus maniculatus (BW) yield parent-of-origin effects on both embryonic and placental growth. The two species are approximately the same size, but a female BW crossed with a male PO produces offspring that are smaller than either parent. In
Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11805345
'Phytoene synthase from the bacterium Erwinia uredovora (crtB) has been overexpressed in tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig). Fruit-specific expression was achieved by using the tomato polygalacturonase promoter, and the CRTB protein was targeted to the chromoplast by the tomato phytoene synthase-1 transit sequence. Total fruit carotenoids of primary
Reduction of false positives in prokaryotic mRNA differential display [In Process Citation]
Journal:Biotechniques
PubMed ID:10343902
Mutations in a new gene in Ellis-van Creveld syndrome and Weyers acrodental dysostosis.
Journal:Nature Genetics
PubMed ID:10700184
Ellis-van Creveld syndrome (EvC, MIM 225500) is an autosomal recessive skeletal dysplasia characterized by short limbs, short ribs, postaxial polydactyly and dysplastic nails and teeth. Congenital cardiac defects, most commonly a defect of primary atrial septation producing a common atrium, occur in 60% of affected individuals. The disease was mapped
Immunogene therapy of tumors with vaccine based on Xenopus homologous vascular endothelial growth factor as a model antigen.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11553767
Overcoming immune tolerance of the growth factors associated with tumor growth should be a useful approach to cancer therapy by active immunity. We used vascular endothelial growth factor (VEGF) as a model antigen to explore the feasibility of the immunogene tumor therapy with a vaccine based on a single xenogeneic