TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells
TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells
Invitrogen™

TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

TA Cloning™ Kit with pCR™2.1 vector는 Taq-증폭 PCR 산물을 plasmid vector에 직접 삽입하는 빠른 원스텝 클로닝 전략을 제공합니다. TA Cloning™자세히 알아보기
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카탈로그 번호반응 수
K20202020 Reactions
K20204040 Reactions
카탈로그 번호 K202020
제품 가격(KRW)
288,000
キャンペーン価格
Ends: 31-Mar-2026
329,000
할인액 41,000 (12%)
Each
카트에 추가하기
반응 수:
20 Reactions
제품 가격(KRW)
288,000
キャンペーン価格
Ends: 31-Mar-2026
329,000
할인액 41,000 (12%)
Each
카트에 추가하기
TA Cloning™ Kit with pCR™2.1 vector는 Taq-증폭 PCR 산물을 plasmid vector에 직접 삽입하는 빠른 원스텝 클로닝 전략을 제공합니다. TA Cloning™ Kit는 pCR™2.1 cloning vector와 ExpressLink™ T4 DNA Ligase를 사용해 15분 내에 실온 ligation 단계에서 ligation 산물을 생성합니다. 일반적으로 반응이후 insert를 포함한 recombinant >80%를 획득합니다.

TA Cloning™ Kit with pCR™2.1 vector의 특징
• 신속성 및 편리성—15분, 실온 ligation
• 효율적—white/blue 스크리닝, 올바르게 삽입된 클론 > 80%
• 유연—유연한 항생제 선택을 위한 kanamycin 또는 ampicillin 내성 선택
• Hassle-free— PCR 산물의 모든 효소 처리 과정들이 필요 없습니다.
• 간편—제한부위를 포함한 PCR primer가 필요하지 않습니다.

pCR™2.1 vector는 다음을 제공합니다.
• Taq-증폭 PCR 산물의 직접 ligation을 위한 3'-T overhang
• in vitro RNA transcription 및 sequencing을 위한 T7 promoter
• insert의 편리한 절제를 위한 flanking EcoR I 부위의 polylinker
• Sequencing을 위한 M13 forward 및 reverse primer site

TA Cloning™ 작업 방식
Taq polymerase는 non-template-비의존성 활성을 가지며 이 때문에 단일 deoxyadenosine (A)를 PCR 산물의 3' end에 첨가합니다. 이 키트에 제공되는 선형 vector에는 단일 3' deoxythymidine (T) residue가 있습니다. 이로 PCR insert를 vector에 효율적으로 접합할 수 있습니다.

키트 구성
TA Cloning™ Kit는 다양한 구성으로 제공됩니다.Competent cell 불포함(K2020-20 및 K2020-40), One Shot™ INVF' Chemically Competent E. coli 포함(K2000-01 및 K2000-40), One Shot™ TOP10F' Chemically Competent E. coli 포함(K2030-01 및 K2030-40), One Shot™ TOP10 Chemically Competent E. coli 포함(K2040-01 및 K2040-40), 20회 반응 및 40회 반응 키트로 제공됩니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주Not Included
클로닝 방법TA Cloning
용도(애플리케이션)PCR Cloning
반응 수20 Reactions
제품라인TA Cloning
제품 유형Cloning Kit
프로모터T7
수량20 reactions
벡터pCR2.1
형식Kit
Unit SizeEach
구성 및 보관
TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls.

Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

How do you recommend that I prepare my DNA for successful electroporation of E. coli?

For best results, DNA used in electroporation must have a very low ionic strength and a high resistance. A high-salt DNA sample may be purified by either ethanol precipitation or dialysis.

The following suggested protocols are for ligation reactions of 20ul. The volumes may be adjusted to suit the amount being prepared.

Purifying DNA by Precipitation: Add 5 to 10 ug of tRNA to a 20ul ligation reaction. Adjust the solution to 2.5 M in ammonium acetate using a 7.5 M ammonium acetate stock solution. Mix well. Add two volumes of 100 % ethanol. Centrifuge at 12,000 x g for 15 min at 4C. Remove the supernatant with a micropipet. Wash the pellet with 60ul of 70% ethanol. Centrifuge at 12,000 x g for 15 min at room temperature. Remove the supernatant with a micropipet. Air dry the pellet. Resuspend the DNA in 0.5X TE buffer [5 mM Tris-HCl, 0.5 mM EDTA (pH 7.5)] to a concentration of 10 ng/ul of DNA. Use 1 ul per transformation of 20 ul of cell suspension.

Purifying DNA by Microdialysis: Float a Millipore filter, type VS 0.025 um, on a pool of 0.5X TE buffer (or 10% glycerol) in a small plastic container. Place 20ul of the DNA solution as a drop on top of the filter. Incubate at room temperature for several hours. Withdraw the DNA drop from the filter and place it in a polypropylene microcentrifuge tube. Use 1ul of this DNA for each electrotransformation reaction.

When should DMSO, formamide, glycerol and other cosolvents be used in PCR?

Cosolvents may be used when there is a failure of amplification, either because the template contains stable hairpin-loops or the region of amplification is GC-rich. Keep in mind that all of these cosolvents have the effect of lowering enzyme activity, which will decrease amplification yield. For more information see P Landre et al (1995). The use of co-solvents to enhance amplification by the polymerase chain reaction. In: PCR Strategies, edited by MA Innis, DH Gelfand, JJ Sninsky. Academic Press, San Diego, CA, pp. 3-16.

Additionally, when amplifying very long PCR fragments (greater than 5 kb) the use of cosolvents is often recommended to help compensate for the increased melting temperature of these fragments.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.