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ViraPower™ II Lentiviral Gateway™ Expression System
The ViraPower™ II Lentiviral Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K36720 | 1 kit |
카탈로그 번호 K36720
제품 가격(KRW)
7,451,000
Each
수량:
1 kit
제품 가격(KRW)
7,451,000
Each
The ViraPower™ II Lentiviral Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ II Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.2⁄V5-DEST™ vector in the kit has the CMV promoter for driving constitutive expression of the target gene in mammalian cells. The ViraPower™ II Lentiviral system uses the PGK promoter instead of SV40 promoter in the lentiviral vector for driving long-term, persistent expression of the blasticidin stable selection marker.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable expression, long term expression
Key Features
• CMV promoter for high level, constitutive expression of the target gene
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit (Cat # V36820)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable expression, long term expression
Key Features
• CMV promoter for high level, constitutive expression of the target gene
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit (Cat # V36820)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템Constitutive
배달 유형Lentiviral
용도(애플리케이션)Viral Expression
제품 유형Lentiviral Expression System
수량1 kit
선택 제제(진핵)Blasticidin
벡터pLenti
클로닝 방법Gateway
제품라인Gateway, ViraPower
프로모터CMV
단백질 태그V5 Epitope Tag
Unit SizeEach
구성 및 보관
pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit
• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)
• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)
• One Shot™ Stbl3™ Chemically Competent E. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).
ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)
• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)
• 50 mg of Blasticidin powder (Cat # R21001)
• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.
Storage
• Vectors: -20°C
• ViraPower™ Packaging Mix: -20°C
• Blasticidin: -20°C
• Lipofectamine™ 2000: 4°C (do not freeze)
• One Shot™ Stbl3™ Chemically Competent E. coli:-80°C
• 293 FT Cells: Liquid Nitrogen
• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)
• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)
• One Shot™ Stbl3™ Chemically Competent E. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).
ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)
• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)
• 50 mg of Blasticidin powder (Cat # R21001)
• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.
Storage
• Vectors: -20°C
• ViraPower™ Packaging Mix: -20°C
• Blasticidin: -20°C
• Lipofectamine™ 2000: 4°C (do not freeze)
• One Shot™ Stbl3™ Chemically Competent E. coli:-80°C
• 293 FT Cells: Liquid Nitrogen