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Invitrogen™
pBAD202 Directional TOPO™ Expression Kit
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K420201 | 20 reactions |
카탈로그 번호 K420201
제품 가격(KRW)
1,359,000
온라인 행사
Ends: 31-Mar-2026
1,598,000할인액 239,000 (15%)
Each
수량:
20 reactions
제품 가격(KRW)
1,359,000
온라인 행사
Ends: 31-Mar-2026
1,598,000할인액 239,000 (15%)
Each
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert (Figure 1A), especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.
In addition to being a more effective way to clone, TOPO™ Cloning eliminates these potential expression problems. TOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO™ cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO™ Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
In addition to being a more effective way to clone, TOPO™ Cloning eliminates these potential expression problems. TOPO™ Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO™ cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO™ Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
For Research Use Only. Not for use in diagnostic procedures.
사양
항생제 내성 박테리아Kanamycin (KanR)
박테리아 또는 효모 균주LMG194, TOP10
분열EK (Enterokinase) Recognition Site
구성 또는 유도성 시스템Inducible
발현 메커니즘Cell-Based Expression
발현 시스템E. coli
유도제Arabinose
제품 유형TOPO Expression Kit
수량20 reactions
선택 제제(진핵)None
벡터pBAD
클로닝 방법Directional TOPO
제품라인TOPO
프로모터araBAD
단백질 태그His Tag (6x), V5 Epitope Tag, Thioredoxin
Unit SizeEach