TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli
본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기
TOPO&trade; TA Cloning&trade; Kit, with One Shot&trade; MAX Efficiency&trade; DH5&alpha;-T1<sup>R</sup> <i>E. coli</i>
인용 및 참조 문헌 (1)
Invitrogen™

TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli

TOPO™ TA Cloning™ Kit는 단 5분 내에 직접적으로 PCR 산물을 클로닝하도록 만들어졌습니다(1). 이들은 pCR™-TOPO™ Vector, 공유결합 topoisomerase I를 사용해 클로닝과자세히 알아보기
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
K45200125 Reactions
K45204050 Reactions
카탈로그 번호 K452001
제품 가격(KRW)
-
수량:
25 Reactions
TOPO™ TA Cloning™ Kit는 단 5분 내에 직접적으로 PCR 산물을 클로닝하도록 만들어졌습니다(1). 이들은 pCR™-TOPO™ Vector, 공유결합 topoisomerase I를 사용해 클로닝과 재조합을 신속하게 합니다. pCR™-TOPO™ Vectors 구성:

• 3´-T overhangs- Taq-증폭 PCR 산물 직접 ligation
• 선택한 T7 (pCR™2.1-TOPO™) 또는 T7 및 SP6 (pCR™II-TOPO™) promoter - in vitro RNA transcription 및 sequencing.각 vector에는 sequencing을 위해 M13 forward 및 reverse primer site도 있습니다.
• EcoR I site flanking - PCR 산물 삽입 또는 쉬운 삽입부 절단
• 선택한 E. coli에 대한 Kanamycin 및 ampicillin 내성 유전자
• 선택한 재조합물에 대한 쉬운 blue/white colony screening

TOPO™ TA Cloning™ Kit는 PureLink™ Quick Plasmid Miniprep Kit (50 preps)와 통합된combo kit로도 이용할 수 있어 차후 분석을 위해 TOPO™ vector에 insert가 포함된 plasmid를 신속하게 정제할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주DH5α
클로닝 방법TOPO™-TA
제품라인One Shot
제품 유형Cloning Kit
프로모터T7, SP6
수량25 Reactions
벡터TOPO-TA Cloning Vectors
형식Kit
Unit SizeEach
구성 및 보관
Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

What is the difference between the pCR2.1-TOPO and pCR4-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCR4-TOPO vector has sequencing primer sites located as close as 33 base pairs from the PCR product insertion site. This minimizes the amount of vector DNA sequence that needs to be read before reaching the sequence of the insert, making the pCR4-TOPO vector very useful for sequencing applications.

What is the difference between the pCR2.1-TOPO and pCRII-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCRII-TOPO vector is a dual promoter vector, containing the SP6 and T7 promoters for in vitro transcription/sequencing, whereas the pCR2.1-TOPO vector contains only the T7 promoter for in vitro transcription/sequencing. Both vectors contain the M13 Forward and Reverse primer sites for sequencing or PCR screening.

Your TOPO cloning kits contain a control template and control primers. Can I obtain the sequence of the control template?

The sequence of the control template is proprietary.

View all TOPO™ TA Cloning™ Kit, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli, 25 Reactions FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
Authors: Olson J H; Xiang X; Ziegert T; Kittelson A; Rawls A; Bieber A L; Chandler D E;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11562501
Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in ... More