TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli

본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기

TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ <i>E. coli</i>

인용 및 참조 문헌 (6)

Invitrogen™

TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli

Name: TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli
SKU: K456001
Price: 875000 KRW

TOPO™ TA Cloning™ Kit는 단 5분 내에 PCR 반응에서 직접적으로 PCR 산물을 클로닝하도록 만들어졌습니다(1). 이들은 pCR™-TOPO™ Vector, 공유결합 topoisomerase I를자세히 알아보기

보기 방식 변경

카탈로그 번호	수량
K456001	25 Reactions
K456040	50 Reactions

2 개 옵션

카탈로그 번호 K456001

제품 가격(KRW)

875,000

온라인 행사

Ends: 31-Mar-2026

1,029,000

할인액 154,000 (15%)

Each

카트에 추가하기

수량:

25 Reactions

제품 가격(KRW)

875,000

온라인 행사

Ends: 31-Mar-2026

1,029,000

할인액 154,000 (15%)

Each

카트에 추가하기

TOPO™ TA Cloning™ Kit는 단 5분 내에 PCR 반응에서 직접적으로 PCR 산물을 클로닝하도록 만들어졌습니다(1). 이들은 pCR™-TOPO™ Vector, 공유결합 topoisomerase I를 사용해 클로닝과 재조합을 신속하게 합니다. pCR™-TOPO™ Vectors 구성:

• 3´-T overhangs- Taq-증폭 PCR 산물을 직접 ligation
• 선택한 T7 (pCR™2.1-TOPO™) 또는 T7 및 SP6 (pCR™II-TOPO™) promoter - in vitro RNA transcription 및 sequencing. 각 vector에는 sequencing을 위해 M13 forward 및 reverse primer site도 있습니다.
• EcoR I site flanking - PCR 산물 삽입 또는 쉬운 삽입부 절단
• 선택한 E. coli에 대한 Kanamycin 및 ampicillin 내성 유전자
• 선택한 재조합체에 대한 손쉬운 blue/white colony screening

TOPO™ TA Cloning™ Kit는 PureLink™ Quick Plasmid Miniprep Kit (50 preps)와 통합된combo kit로도 이용할 수 있어 차후 분석에 TOPO™-클론 삽입부를 신속하게 plasmid 정제할 수 있습니다.

For Research Use Only. Not for use in diagnostic procedures.

사양

박테리아 또는 효모 균주TOP10

클로닝 방법TOPO™-TA

제품라인One Shot

제품 유형Cloning Kit

수량25 Reactions

벡터TOPO-TA Cloning Vectors

형식Kit

Unit SizeEach

구성 및 보관

Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

What is the difference between the pCR2.1-TOPO and pCR4-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCR4-TOPO vector has sequencing primer sites located as close as 33 base pairs from the PCR product insertion site. This minimizes the amount of vector DNA sequence that needs to be read before reaching the sequence of the insert, making the pCR4-TOPO vector very useful for sequencing applications.

What is the difference between the pCR2.1-TOPO and pCRII-TOPO vectors?

The vector backbones for both of these vectors are very similar. The main difference is that the pCRII-TOPO vector is a dual promoter vector, containing the SP6 and T7 promoters for in vitro transcription/sequencing, whereas the pCR2.1-TOPO vector contains only the T7 promoter for in vitro transcription/sequencing. Both vectors contain the M13 Forward and Reverse primer sites for sequencing or PCR screening.

Your TOPO cloning kits contain a control template and control primers. Can I obtain the sequence of the control template?

The sequence of the control template is proprietary.

View all TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli, 25 Reactions FAQs

인용 및 참조 문헌 (6)

인용 및 참조 문헌

Abstract

SDF-1a induces PDGF-B expression and the differentiation of bone marrow cells into pericytes.

Authors:Hamdan R, Zhou Z, Kleinerman ES

Journal:Mol Cancer Res

PubMed ID:21911740

'Platelet-derived growth factor B (PDGF-B) and its receptor, PDGFR-ß, play a critical role in pericyte maturation; however, the mechanisms by which PDGF-B is upregulated in the tumor microenvironment remain unclear. We previously showed that upregulating stromal-derived factor, SDF-1a, in VEGF(165)-inhibited Ewing''s sarcoma tumors (TC/siVEGF(7-1)) induced PDGF-B mRNA expression, increased infiltration ... More

Genetic engineering of phytochrome biosynthesis in bacteria.

Authors: Gambetta G A; Lagarias J C;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11553807

The bilin prosthetic groups of the phytochrome photoreceptors and the light-harvesting phycobiliprotein antennae arise from the oxygen-dependent ring opening of heme. Two ferredoxin-dependent enzymes contribute to this conversion: a heme oxygenase and a bilin reductase with discrete double-bond specificity. Using a dual plasmid system, one expressing a truncated cyanobacterial apophytochrome ... More

Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.

Authors: Olson J H; Xiang X; Ziegert T; Kittelson A; Rawls A; Bieber A L; Chandler D E;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11562501

Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in ... More

Determination of the loss of function complement C4 exon 29 CT insertion using a novel paralog-specific assay in healthy UK and Spanish populations.

Authors:Boteva L, Wu YL, Cortes-Hernández J, Martin J, Vyse TJ, Fernando MM

Journal:PLoS One

PubMed ID:21857912

Genetic variants resulting in non-expression of complement C4A and C4B genes are common in healthy European populations and have shown association with a number of diseases, most notably the autoimmune disease, systemic lupus erythematosus. The most frequent cause of a C4 "null" allele, following that of C4 gene copy number ... More

Identification of seven haplotypes of the caprine PrP gene at codons 127, 142, 154, 211, 222 and 240 in French Alpine and Saanen breeds and their association with classical scrapie.

Authors:Barillet F, Mariat D, Amigues Y, Faugeras R, Caillat H, Moazami-Goudarzi K, Rupp R, Babilliot JM, Lacroux C, Lugan S, Schelcher F, Chartier C, Corbière F, Andréoletti O, Perrin-Chauvineau C

Journal:J Gen Virol

PubMed ID:19218225

In sheep, susceptibility to scrapie is mainly influenced by polymorphisms of the PrP gene. In goats, there are to date few data related to scrapie susceptibility association with PrP gene polymorphisms. In this study, we first investigated PrP gene polymorphisms of the French Alpine and Saanen breeds. Based on PrP ... More

View all TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 Electrocomp™ E. coli, 25 Reactions citations