Search Thermo Fisher Scientific
제품 개요
그림
동영상
관련 제품 추천
관련 제품 추천
문서
FAQ
인용 및 참조 문헌
추가 정보
관련 제품 추천
TOPO™ TA Cloning™ Kits are designed for cloning PCR products directly from a PCR reaction in just 5 minutes (1). They use a pCR™-TOPO™ Vector with covalently bound topoisomerase I for fast cloning and recombinants. pCR™-TOPO™ Vectors include:
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR™2.1-TOPO™) or T7 and SP6 (pCR™II-TOPO™) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
TOPO™ TA Cloning™ Kits are also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Choice of T7 (pCR™2.1-TOPO™) or T7 and SP6 (pCR™II-TOPO™) promoters for in vitro RNA transcription and sequencing. Each vector also contains M13 forward and reverse primer sites for sequencing.
• EcoR I sites flanking the PCR product insertion site for easy excision of inserts
• Kanamycin and ampicillin resistance genes for your choice of selection in E. coli
• Easy blue/white colony screening for selection of recombinants
TOPO™ TA Cloning™ Kits are also available in combo kits combined with a PureLink™ Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO™-cloned inserts for downstream analysis.
For Research Use Only. Not for use in diagnostic procedures.
사양
박테리아 또는 효모 균주
DH5α
클로닝 방법
TOPO™-TA
형식
Kit
제품라인
One Shot™
제품 유형
Cloning Kit
프로모터
T7, SP6
수량
50 Reactions
벡터
TOPO-TA Cloning Vectors
구성 및 보관
Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.
Store at -68 to -85°C.
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water
Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.
Store at -68 to -85°C.
그림
문서 및 다운로드
Certificates | 증명서
Lot 번호 또는 부분 Lot 번호로 검색하십시오.
자주 묻는 질문(FAQ)
인용 및 참조 문헌
Search citations by name, author, journal title or abstract text