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Invitrogen™

pGlow TOPO™ TA Expression Kit

The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity.자세히 알아보기

카탈로그 번호	수량
K483001	20 reactions

카탈로그 번호 K483001

제품 가격(KRW)

2,535,000

キャンペーン価格

Ends: 31-Mar-2026

2,982,000

할인액 447,000 (15%)

20 reactions

제품 가격(KRW)

2,535,000

キャンペーン価格

Ends: 31-Mar-2026

2,982,000

할인액 447,000 (15%)

The pGlow TOPO® TA Expression Kit uses the Cycle 3 GFP mutant (1) as a reporter protein for transcriptional activity. Cycle 3 GFP is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.

For Research Use Only. Not for use in diagnostic procedures.

배달 유형Transfection

용도(애플리케이션)Reporter Assays, Clone your own Promoter

제품 유형TOPO TA Expression Kit

수량20 reactions

리포터 유전자GFP (Cycle 3)

선택 제제(진핵)Geneticin™ (G-418)

벡터pGlow, TOPO-TA Vectors

클로닝 방법TOPO-TA

제품라인TOPO

프로모터None (Promoterless)

단백질 태그Untagged

Unit SizeEach

구성 및 보관

pGlow TOPO™ TA Expression Kits contains two boxes. The TOPO™ TA Cloning box contains linearized and topoisomerase I-activated pGlow-TOPO™ vector, dNTPs, primers for sequencing, salt solution, and control PCR template and primers; store at -20°C. The One Shot™ box contains One Shot™ TOP10 E. coli cells, S.O.C. medium, and a supercoiled control plasmid; store at -80°C. The kits are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

Can I use pBlue- or pGlow-TOPO reporter vectors to evaluate promoter function in E. coli?

pBlue-TOPO contains a cryptic prokaryotic promoter upstream of the lacZ reporter gene, due to which E. coli transformants may appear to be light blue when screened on plates containing X-Gal. Hence, we do not recommend using pBlue-TOPO to evaluate promoter function in E. coli. However, pGlow-TOPO can be used for these studies. Note that background expression of beta-galactosidase from pBlue-TOPO does not occur in mammalian cells.

Do you offer a vector that I could use to do some promoter analysis studies?

Yes, we do offer the pBlue-TOPO and pGlow-TOPO vectors that facilitate cloning of the DNA sequence of interest directly upstream of either the b-galactosidase or Cycle 3 GFP gene, respectively.
pBlue-TOPO is ideal for functional analysis of promoters with low transcriptional activity, since assays for beta-galactosidase are easy to perform and are quantitative at very low levels of expression. pGlow-TOPO is ideal for non-invasive analysis of promoter elements within intact, living cells. The fluorescent property of Cycle 3 GFP allows in vivo detection in virtually any cell type or species using microscopy with wild-type GFP filter sets or by fluorescence-activated cell sorting methods.

Can GFP fluorescence be detected in cells that have been stained for beta-galactosidase?

We recommend looking for GFP fluorescence before staining for beta-galactosidase. This is because the beta-galactosidase staining process produces a very high autofluorescence that will interfere with detection of GFP fluorescence.

View all pGlow TOPO™ TA Expression Kit FAQs