DES™-Inducible Kit with pCoBlast

The DES™-Inducible Kit provides the expression vector pMT/V5-His and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector자세히 알아보기

카탈로그 번호	수량
K512001	1 kit

카탈로그 번호 K512001

제품 가격(KRW)

1 kit

The DES™-Inducible Kit provides the expression vector pMT/V5-His and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium. The pMT/V5-His vector offers the following features:

• Small size (3.5 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogen's Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin

To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.

For Research Use Only. Not for use in diagnostic procedures.

제품 유형Inducible Kit

단백질 태그 위치(유전자에 대해)C-terminal

수량1 kit

벡터pMT

클로닝 방법Gateway

제품라인DES

프로모터MT

단백질 태그His Tag (6x), V5 Epitope Tag

Unit SizeEach

구성 및 보관

Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider's Drosophila Medium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.

자주 묻는 질문(FAQ)

What is the doubling time of Drosophila S2 cells?

When grown at the optimal temperature of 27-28ºC, Drosophila S2 cells grow very quickly, with an approximate 14-16 hr doubling time. The cells can also be grown at 22-25ºC with doubling time of 24hrs. Humidity is an issue, so a shallow pan of water can be left in the incubator, but the incubator does not need to be turned on.

Growth medium is also a factor in doubling time - the doubling times listed here are for Sf900 II medium.

The cells can reach maximum densities of 1 to 2.5 x 10E7 cells/ml in shake flask cultures.

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

A novel allelic variant of the human TSG-6 gene encoding an amino acid difference in the CUB module. Chromosomal localization, frequency analysis, modeling, and expression.

Authors: Nentwich Hilke A; Mustafa Zehra; Rugg Marilyn S; Marsden Brian D; Cordell Martin R; Mahoney David J; Jenkins Suzanne C; Dowling Barbara; Fries Erik; Milner Caroline M; Loughlin John; Day Anthony J;

Journal:J Biol Chem

PubMed ID:11854277

Tumor necrosis factor-stimulated gene-6 (TSG-6) encodes a 35-kDa protein, which is comprised of contiguous Link and CUB modules. TSG-6 protein has been detected in the articular joints of osteoarthritis (OA) patients, with little or no constitutive expression in normal adult tissues. It interacts with components of cartilage matrix (e.g. hyaluronan ... More