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Invitrogen™
DES™-Inducible/Secreted Kit with pCoBlast
The DES™-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast)자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K513001 | 1 kit |
카탈로그 번호 K513001
제품 가격(KRW)
5,331,000
線上優惠
Ends: 31-Dec-2025
6,271,000할인액 940,000 (15%)
Each
수량:
1 kit
제품 가격(KRW)
5,331,000
線上優惠
Ends: 31-Dec-2025
6,271,000할인액 940,000 (15%)
Each
The DES™-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or hygromycin (pCoHygro) selection. This vector offers the inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride. The N-terminal signal sequence from the insect BiP gene is provided to direct the recombinant fusion protein through the secretory pathway of S2 cells into the culture medium. The pMT/BiP/V5-His vector offers the following additional features:
• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.
• Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogens Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the BiP signal sequence.
For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형Inducible/Secreted Kit
단백질 태그 위치(유전자에 대해)C-terminal
수량1 kit
벡터pMT
클로닝 방법Gateway
제품라인DES
프로모터MT
단백질 태그His Tag (6x), V5 Epitope Tag
Unit SizeEach
구성 및 보관
The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider's Drosophila Medium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.