본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기

Invitrogen™

pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

카탈로그 번호	수량
K531520 K5315-20으로도 사용됨	1 kit

카탈로그 번호 K531520

K5315-20으로도 사용됨

제품 가격(KRW)

3,474,000

Exklusiv online

Ends: 31-Mar-2026

4,087,000

할인액 613,000 (15%)

1 kit

제품 가격(KRW)

3,474,000

Exklusiv online

Ends: 31-Mar-2026

4,087,000

할인액 613,000 (15%)

Invitrogen's™ pLenti6.3⁄V5™-TOPO™Cloning Kit는 ViraPower™ HiPerform™ Lentiviral Expression System (카탈로그 K5310-00)의 일부입니다.

pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit에는 분할(dividing) 및 비분할(non-dividing) 포유류 세포에서 표적 유전자의 신속한 PCR 기반 클로닝과 높은 수준의 발현을 위한 TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO™가 들어있습니다. pLenti6.3⁄V5™-TOPO™ vector는 두 가지 핵심 유전 염기서열을 갖추고 있는 HiPerform™ vector입니다. HIV-1 integrase 유전자의 Woodchuck Posttranscriptional Regulatory Element (WPRE)와 central Polypurine Tract (cPPT) sequence는 이런 염기서열이 없는 vector보다 단백질 발현이 4배 이상 높습니다.

장점
• 안정적인 발현
• 장기 실험
• 기능성 바이러스의 정확한 역가
• 단 3단계의 5분, 고효율 TOPO™ PCR 클로닝

주요 특징
• HiPerform™ WPRE 및 cPPT 성분
• CMV promoter
• C 말단의 V5 epitope tag
• Blasticidin 선택

Kit 구성
• 양성 control로 lacZ vector, pLenti6.3⁄V5-GW⁄lacZ™
• Stbl3™ competent cells

연구용으로만 사용할 수 있습니다. 치료 또는 진단 목적으로 사용할 수 없습니다.

For Research Use Only. Not for use in diagnostic procedures.

세포 유형Competent Cell

클로닝 방법TOPO-TA

구성 또는 유도성 시스템Constitutive

배달 유형Lentiviral

발현 시스템ViraPower™ HiPerform™ Lentiviral Expression Systems

용도(애플리케이션)Protein Expression

주요 기능Viral Expression

제품라인HiPerform, TOPO, ViraPower

제품 유형Cloning Kit

프로모터CMV

단백질 태그V5 Epitope Tag

수량1 kit

선택 제제(진핵)Blasticidin

선택 마커 프로모터SV40 Promoter

벡터pLenti, TOPO-TA Vector

형식Kit

Unit SizeEach

구성 및 보관

TOPO™ TA Cloning Reagents (Box 1)
pLenti6.3?V5™-TOPO™ (5-10 ng⁄μL): 20 μL, -20°C
pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 μg⁄μL): 20 μL, -20°C
10X PCR Buffer: 100 μL, -20°C
dNTP Mix: 10 μL, -20°C
Salt Solution: 50 μL, -20°C
CMV Forward Primer (100 ng⁄μL): 20 μL, -20°C
V5 (C-term) Reverse Primer (100 ng⁄μL): 20 μL, -20°C
Control PCR Template (50 ng⁄μL): 10 μL, -20°C
Control PCR Primers (100 ng⁄μL): 10 μL, each -20°C
Sterile Water: 1 mL

One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
Stbl3™ Cells: 21 x 50 μL, -80°C
pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
S.O.C. Medium: 6 mL, -80°C

자주 묻는 질문(FAQ)

Can I store my competent E. coli in liquid nitrogen?

We do not recommend storing competent E. coli strains in liquid nitrogen as the extreme temperature can be harmful to the cells. Also, the plastic storage vials are not intended to withstand the extreme temperature and may crack or break.

How should I store my competent E. coli?

We recommend storing our competent E. coli strains at -80°C. Storage at warmer temperatures, even for a brief period of time, will significantly decrease transformation efficiency.

What is the best molar ratio of PCR product:vector to use for TOPO TA cloning? Is there an equation to calculate the quantity to use?

We suggest starting with a molar ratio of 1:1 (insert:vector), with a range of 0.5:1 to 2:1. The quantity used in a TOPO cloning reaction is typically 5-10 ng of a 2 kb PCR product.

Equation:

length of insert (bp)/length of vector (bp) x ng of vector = ng of insert needed for 1:1 (insert:vector ratio)

What is the best ratio of insert:vector to use for cloning? Is there an equation to calculate this?

The optimal ratio is 1:1 insert to vector. Optimization can be done using a ratio of 0.5-2 molecules of insert for every molecule of the vector.

Equation:

length of insert (bp)/length of vector (bp) x ng of vector = ng of insert needed for 1:1 insert:vector ratio

Does Platinum Taq DNA Polymerase High Fidelity enzyme mix leave 3' A-overhangs on the PCR product for subsequent cloning into a TOPO TA or original TA vector?

Yes, the enzyme mix leaves 3' A-overhangs on a portion of the PCR products. However, the cloning efficiency is greatly decreased compared to that obtained with Taq polymerase alone. It is recommended to add 3' A-overhangs to the product for TA cloning.

View all pLenti6.3/V5™-TOPO™ TA Cloning™ Kit FAQs