MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli자세히 알아보기
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카탈로그 번호
수량
K6803
1 L
카탈로그 번호 K6803
제품 가격(KRW)
176,000
온라인 행사
Ends: 31-Dec-2025
207,000
할인액 31,000 (15%)
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수량:
1 L
제품 가격(KRW)
176,000
온라인 행사
Ends: 31-Dec-2025
207,000
할인액 31,000 (15%)
Each
카트에 추가하기
MagicMedia™ E. coli Expression Medium is specifically designed to dramatically increase the yield of recombinant proteins in T7-regulated E. coli expression systems without monitoring culture O.D. (optical densities) or adding inducer. The proprietary MagicMedia™ formulation enables E. coli growth to reach culture densities 3-10-fold higher than those achieved with traditional LB + IPTG methods, allowing you to:
• Achieve 3-10 times higher protein yields (Figure 1) with much less hands-on time • Eliminate time-consuming O.D. monitoring steps-simply inoculate prepared MagicMedia™ E. coli Expression Medium, grow the culture overnight, and harvest (Figure 2) • Reduce culture volume to simplify handling and scale-up processes
In addition, MagicMedia™ E. coli Expression Medium is ideal for simultaneous expression of multiple clones for high-throughput applications.
For Research Use Only. Not for use in diagnostic procedures.
사양
형식Liquid
제품 유형E. Coli Expression Medium
수량1 L
배송 조건Room Temperature
타겟 유기체 분류E. coli
제품라인MagicMedia
Unit SizeEach
구성 및 보관
Component A (1X liquid) and Component B (liquid)
Store in the dark at 2−8°C.
Shelf life: 12 months from date of manufacture
자주 묻는 질문(FAQ)
Can I use a lower incubation temperature for MagicMedia Medium?
Yes, expression at 18 degrees C and 25 degrees C has been validated using the Dual Temperature expression protocol.
I am using a Champion pET vector with the BL21-AI cells. Can I use your MagicMedia Medium for expression?
Unfortunately, MagicMedia Medium will not work with the arabinose inducer. MagicMedia Medium needs an IPTG-inducible system along with an intact Lac operon.
MagicMedia Medium is an E. coli expression medium. It produces 3-10 times higher protein yield, is compatible with T7 regulated E. coli vectors that contain a functional lac operon and BL21 (DE3) strains, is simple to use, and eliminates OD monitoring/induction steps.
How does expression in MagicMedia affect the solubility of proteins when compared to standard LB and IPTG induction?
In most cases, the ratio of soluble to insoluble proteins is not much different when compared to LB and IPTG. However, since more total protein is typically obtained in MagicMedia, one also obtains more total soluble protein.
How does MagicMedia compare to LB-IPTG with regards to expression of toxic proteins?
Toxic protein expression has not been investigated using MagicMedia. However, the Dual Temperature protocol provided in the manual can be tried. This may be a situation where LB/IPTG induction has an advantage since parameters (time, temperature, and IPTG concentration) are easily adjusted for optimal expression.
Diversity in tissue expression, substrate binding and SCF complex formation for a lectin family of ubiquitin ligases.
Authors:Glenn KA, Nelson RF, Wen HM, Mallinger AJ, Paulson HL,
Journal:J Biol Chem
PubMed ID:18203720
'Post-translational modification of proteins regulates many cellular processes. Some modifications, including N-linked glycosylation, serve multiple functions. For example, the attachment of N-linked glycans to nascent proteins in the endoplasmic reticulum (ER) facilitates proper folding, while retention of high mannose glycans on misfolded glycoproteins serves as a signal for retrotranslocation and ... More