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인용 및 참조 문헌 (2)
Invitrogen™
InsectSelect™ Glow Kit with Sf9 Cells
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| K81001 K810-01으로도 사용됨 | 1 kit |
카탈로그 번호 K81001
K810-01으로도 사용됨
제품 가격(KRW)
-
수량:
1 kit
The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of your
gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
gene of interest. This vector has the following features:
• The OpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
In addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistance
protein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lines
with Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형Sf9 Cells Glow Kit
단백질 태그 위치(유전자에 대해)C-terminal
수량1 kit
벡터pIZ
클로닝 방법Restriction Enzyme/MCS
제품라인InsectSelect
프로모터OplE2
단백질 태그His Tag (6x), V5 Epitope Tag
Unit SizeEach
구성 및 보관
The InsectSelect™ Glow Kit includes 20 μg each of supercoiled, lyophilized pIZT/V5-His and pIZT/V5-His/CAT, 2 μg each of lyophilized OpIE2 Forward and Reverse Sequencing primers, 1 g Zeocin,™ Cellfectin™ Reagent, Sf9 cells, and 1 L of GIBCO™ Grace's Insect Media. The pIZT/V5-His Vector Kit contains only the expression vectors and primers as described above. Store frozen insect cells in liquid nitrogen. Store medium and Cellfectin™ Reagent at +4°C. Store all other reagents at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
자주 묻는 질문(FAQ)
Does the incubator need to be humidified when culturing insect cells?
인용 및 참조 문헌 (2)
인용 및 참조 문헌
Abstract
Disarming the mustard oil bomb.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12161563
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called
Punctin, a novel ADAMTS-like molecule, ADAMTSL-1, in extracellular matrix.
Journal:J Biol Chem
PubMed ID:11805097
Punctin (ADAMTSL-1) is a secreted molecule resembling members of the ADAMTS family of proteases. Punctin lacks the pro-metalloprotease and the disintegrin-like domain typical of this family but contains other ADAMTS domains in precise order including four thrombospondin type I repeats. Punctin is the product of a distinct gene on human