The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit자세히 알아보기
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카탈로그 번호
수량
K95001
6 purifications
카탈로그 번호 K95001
제품 가격(KRW)
1,096,000
Online offer
Ends: 31-Mar-2026
1,289,000
할인액 193,000 (15%)
Each
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수량:
6 purifications
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
1,096,000
Online offer
Ends: 31-Mar-2026
1,289,000
할인액 193,000 (15%)
Each
카트에 추가하기
The Ni-NTA Purification System is designed for the purification of recombinant proteins that contain a polyhistidine (6xHis) sequence. The kit utilizes Ni-NTA nickel-chelating resin and is supplied with native and denaturing buffers for efficient purification of recombinant proteins under different conditions.
For Research Use Only. Not for use in diagnostic procedures.
사양
형식Suspension
제품 유형Ni-NTA Purification System
수량6 purifications
단백질 태그His Tag
Unit SizeEach
구성 및 보관
Six 2-ml resin columns and buffers for native and denaturing purification. Ten milliliters of Ni-NTA Agarose. Store at +4°C. All reagents are guaranteed stable for 6 months when properly stored.
자주 묻는 질문(FAQ)
Can ProBond or Ni-NTA beads be used for large-scale preparations?
ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).
A composite Ets/Pit-1 binding site in the prolactin gene can mediate transcriptional responses to multiple signal transduction pathways.
Authors:Howard PW, Maurer RA
Journal:J Biol Chem
PubMed ID:7673116
'Binding sites for the tissue-specific transcription factor, Pit-1, are required for basal and hormonally induced prolactin gene transcription. Although Pit-1 is phosphorylated in response to several signaling pathways, the mechanism by which Pit-1 contributes to hormonal induction of gene transcription has not been defined. Recent reports suggest that phosphorylation of ... More
Sulfation of N-acetylglucosamine by chondroitin 6-sulfotransferase 2 (GST-5).
Authors: Bhakta S; Bartes A; Bowman K G; Kao W M; Polsky I; Lee J K; Cook B N; Bruehl R E; Rosen S D; Bertozzi C R; Hemmerich S;
Journal:J Biol Chem
PubMed ID:10956661
'Based on sequence homology with a previously cloned human GlcNAc 6-O-sulfotransferase, we have identified an open reading frame (ORF) encoding a novel member of the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family termed GST-5 on the human X chromosome (band Xp11). GST-5 has recently been characterized as a novel GalNAc 6-O-sulfotransferase termed chondroitin ... More
Probable Identification of a Membrane-Associated Repressor of Bacillus subtilis DNA Replication as the E2 Subunit of the Pyruvate Dehydrogenase Complex
Authors:Andrew Stein and William Firshein*
Journal:J Bacteriol
PubMed ID:10735853
Two Bacillus subtilis lysogenic libraries were probed by an antibody specific for apreviously described membrane-associated inhibitor of B. subtilis DNA replication(J. Laffan and W. Firshein, Proc. Natl. Acad. Sci. USA 85:7452-7456, 1988). Threeclones that reacted strongly with the antibody contained an entire open reading frame.Sequencing identified one of the clones ... More
Sox9 and p300 cooperatively regulate chromatin-mediated transcription.
Authors:Furumatsu T, Tsuda M, Yoshida K, Taniguchi N, Ito T, Hashimoto M, Ito T, Asahara H,
Journal:J Biol Chem
PubMed ID:16109717
Chromatin structure is a fundamental component of gene regulation, expression, and cellular differentiation. We have previously reported that the multifunctional coactivator p300 is a member of the Sox9-related transcriptional apparatus and activates Sox9 (Sry-type high-mobility-group box 9)-dependent transcription during chondrogenesis. However, the mechanism of synergy between Sox9 and p300 in ... More