LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
인용 및 참조 문헌 (210)
Invitrogen™

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells

LIVE⁄DEAD™ Viability⁄Cytotoxicity Kit는 혈장막 integrity과 esterase 활성에 기초해 집단 내 세포 생존율을 판단할 수 있는 빠르고 쉬운 two-color assay 입니다.이자세히 알아보기
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카탈로그 번호수량
L32241 kit
카탈로그 번호 L3224
제품 가격(KRW)
852,000
온라인 행사
Ends: 31-Dec-2025
946,000
할인액 94,000 (10%)
Each
카트에 추가하기
수량:
1 kit
제품 가격(KRW)
852,000
온라인 행사
Ends: 31-Dec-2025
946,000
할인액 94,000 (10%)
Each
카트에 추가하기
LIVE⁄DEAD™ Viability⁄Cytotoxicity Kit는 혈장막 integrity과 esterase 활성에 기초해 집단 내 세포 생존율을 판단할 수 있는 빠르고 쉬운 two-color assay 입니다.이 키트는 유세포분석기, 형광현미경, 형광 microplate reader 등에 사용할 수 있습니다.

다른 방법보다 우수한 장점은 다음과 같습니다.
•  신속
•  안전
•  높은 민감성
•  적정한 가격

다양한 기술과 세포 유형에 쉽게 사용 가능함
편재되어 있는 세포 간 esterase 활성과 완전한 혈장막이 살아있는 세포의 두드러진 특징입니다. LIVE⁄DEAD™ Viability⁄Cytotoxicity Kit는 세포 간 esterase 활성을 제시하는 green-fluorescent calcein-AM 염색과 혈장막 integrity의 손실 여부를 확인 할 수 있게 하는 red-fluorescent ethidium homodimer-1 염색을 동시에 실시해 죽은 세포와 살아있는 세포를 신속하게 구분합니다. 이런 특성으로 인해 세포독성 상태가 이런 세포 영향을 만들어 내는 대부분의 진핵세포에 적용가능합니다. 이 분석법은 다양한 형광검출법에 유용합니다.

민감하고 안전하며 효율적
LIVE⁄DEAD™ Viability⁄Cytotoxicity Kit는 죽은 세포 구별에 흔히 사용되는 Trypan blue 제외법보다 민감성이 높습니다. 비용면에서도 효율적인 LIVE⁄DEAD™ Viability⁄Cytotoxicity Kit는 살아있는 세포(calcein-AM) 혹은 죽은 세포(ethidium homodimer-1)와 상호작용을 하며 두가지 모두 염색하는 bridgt fluorescence로 민감성이 높습니다. 두 염색 모두 세포와 상호반응 전에는 실질적으로 형광이 아니기 때문에 background level은 낮습니다.

LIVE⁄DEAD™ Assays 다양한 어플리케이션에 이용 가능함
선정된 Invitrogen LIVE⁄DEAD™ Viability Assays는 포유류 세포, 박테리아, 효모, 진균류에 대해 제공되며 Fixable Dead Cell Stain Kits는 유세포분석을 위한 세포 간 염색에 사용합니다. 모든 LIVE⁄DEAD™ assay가 생존 세포와 비생존 세포를 빠르고 정확하게 구분합니다.

이 제품은 연구용으로만 사용해야 합니다. 동물이나 인간 치료 또는 진단용으로 사용할 수 없습니다.

관련 링크
•  전체 LIVE⁄DEAD™ Assay 제품에 대해 자세하게 알아보세요
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells
설명LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
형식Tube(s), 96-well plate, Slide(s)
수량1 kit
배송 조건Room Temperature
색상Green, Red
Emission517/617
Excitation Wavelength Range494, 528 nm
용도(애플리케이션)Viability Assay
용도 (장비)Fluorescence Microscope, Flow Cytometer, Microplate Reader
제품라인LIVE/DEAD
제품 유형Viability/Cytotoxicity Kit
Unit SizeEach
구성 및 보관
Store in freezer at -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

I need to use a dead cell control for my viability assay. Do you have a protocol for killing cells for this?

Heat killing is commonly used. Place your cells in a tube in buffer and heat at 60oC for 20 minutes. You can also kill your cells by fixing them with ice cold 70% ethanol for 15 minutes. The ethanol-killed cells can then be stored at -20oC until needed, at which point you wash out the ethanol and replace with buffer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am doing a Live/Dead assay using Calcein, AM, for live cells and ethidium homodimer-1 for dead cells. Can I fix the cells after labeling and retain the staining?

This is not recommended. Neither Calcein nor ethidium homodimer-1 bind to any cellular components upon fixation. There is no guarantee that the dyes will be retained upon fixation or any subsequent wash steps. We recommend scoring for live and dead cells as soon as possible after staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store the LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells (Cat. No. L3224)?

We recommend storing the LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells (Cat. No. L3224) in the freezer at -5 degrees C to -30 degrees C and protected from light.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What fluorescent viability assays can I use on the Countess II FL automated cell counter?

We have validated the following kits for use on the Countess II FL Automated Cell Counter:

LIVE/DEAD Viabilty/Cytoxicity Kit (Cat. No. L3224) containing calcein AM and ethidium homodimer-1
ReadyProbes Cell Viability Imaging Kit, Blue/Green (Cat. No. R37609)
ReadyProbes Cell Viability Imaging Kit, Blue/Red (Cat. No. R37610) containing NucBlue Live/NucGreen Dead and NucBlue Live/propidium iodide
See this Application Note for details - https://www.thermofisher.com/content/dam/LifeTech/global/life-sciences/Laboratory%20Instruments/Files/0415/CO014723-Countess-II-FL-Viability-Appnote_FHR.pdf.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (210)

인용 및 참조 문헌
Abstract
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Authors:Jackson WF,Huebner JM,Rusch NJ
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:9110282
OBJECTIVE: The goal of the present study was to develop a method to isolate viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain and ... More
Enzymatic isolation and characterization of single vascular smooth muscle cells from cremasteric arterioles.
Authors:Jackson WF,Huebner JM,Rusch NJ
Journal:Microcirculation (New York, N.Y. : 1994)
PubMed ID:8930888
OBJECTIVE: The goal of the present study was to develop a method to isolate enzymatically viable arteriolar muscle cells from single cremasteric arterioles, which retain the contractile and electrophysiological phenotype of the donor microvessels. METHODS: Arterioles were hand-dissected from rat and hamster cremaster muscles and dissociated by incubation in papain ... More
Calcein: a novel marker for lymphocytes which enter lymph nodes.
Authors:Weston SA, Parish CR
Journal:Cytometry
PubMed ID:1451604
Previous studies have identified unique cell surface antigens which are associated with the specific binding of lymphocytes to high endothelial venules (HEV). Evidence is presented in this paper which demonstrates that uptake of the fluorescent dye calcein by lymphocytes represents an additional marker for the lymph node homing subpopulation of ... More
Successful storage of peripheral nerve before transplantation using green tea polyphenol: an experimental study in rats.
Authors:Ikeguchi R, Kakinoki R, Okamoto T, Matsumoto T, Hyon SH, Nakamura T
Journal:Exp Neurol
PubMed ID:14769360
Green tea polyphenol is known to act as a buffer, reducing biological responses to oxidative stress. Several effects of polyphenol have been reported, such as protection of tissue from ischemia, antineoplasmic and anti-inflammatory effects, and suppression of arteriosclerosis. In this study, we investigated whether peripheral nerve segments could be kept ... More
Human stem cell delivery for treatment of large segmental bone defects.
Authors:Dupont KM, Sharma K, Stevens HY, Boerckel JD, García AJ, Guldberg RE,
Journal:Proc Natl Acad Sci U S A
PubMed ID:20133731
'Local or systemic stem cell delivery has the potential to promote repair of a variety of damaged or degenerated tissues. Although various stem cell sources have been investigated for bone repair, few comparative reports exist, and cellular distribution and viability postimplantation remain key issues. In this study, we quantified the ... More
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