LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
LIVE/DEAD&trade; <i>Funga</i>Light&trade; Yeast Viability Kit, for flow cytometry
인용 및 참조 문헌 (1)
Invitrogen™

LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes자세히 알아보기
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카탈로그 번호수량
L34952200 Assays kit
카탈로그 번호 L34952
제품 가격(KRW)
854,000
キャンペーン価格
Ends: 31-Mar-2026
1,004,000
할인액 150,000 (15%)
Each
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수량:
200 Assays kit
제품 가격(KRW)
854,000
キャンペーン価格
Ends: 31-Mar-2026
1,004,000
할인액 150,000 (15%)
Each
카트에 추가하기

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Impermeant, Permeant
설명LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
검출 방법Fluorescence
염료 유형SYTO™ 9, propidium iodide
형태Solution
형식Tube(s)
수량200 Assays kit
배송 조건Room Temperature
색상Green, Red
Emission498/617
Excitation Wavelength Range485/535 nm
용도(애플리케이션)Viability Assay
용도 (장비)Flow Cytometer
제품라인FungaLight, LIVE/DEAD
제품 유형Yeast Viability Kit
Unit SizeEach
구성 및 보관
Contains 1 vial of SYTO™ 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).

Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

What are the fluorescence excitation/emission maxima for the SYTO 9 dye and propidium iodide in the LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry?

SYTO 9 dye: 480/500 nm
Propidium iodide: 490/635 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I look at bacteria and yeast on a flow cytometer?

Yes, you can. We offer:

-Counting assays: Bacteria Counting Kit, for flow cytometry (Cat. No. B7277) or LIVE/DEAD BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856).
-Viability/vitality assays: LIVE/DEAD BacLight Bacterial Viability Kit (Cat. No. L13152).
-Membrane potential: BacLight Bacterial Membrane Potential Kit (Cat. No. B34950).
-Yeast viability/vitality assays: LIVE/DEAD FungaLight Yeast Viability Kit, for flow cytometry (Cat. No. L34952), FungaLight Yeast CFDA, AM/Propidium Iodide Vitality Kit (Cat. No. F34953)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Quantification of Saccharomyces cerevisiae viability using BacLight.
Authors:Zhang T, Fang HH
Journal:Biotechnol Lett
PubMed ID:15269525
Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified ... More