Search

  • 주문 현황
  • 빠른주문
LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation
인용 및 참조 문헌 (3)
Invitrogen™

LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation

The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
Have Questions?
보기 방식 변경buttonViewtableView
카탈로그 번호수량
L34964400 Assays
L34955200 Assays
L3496380 Assays
카탈로그 번호 L34964
제품 가격(KRW)
557,000
Online offer
Ends: 31-Dec-2026
655,000
할인액 98,000 (15%)
Each
수량:
400 Assays
제품 가격(KRW)
557,000
Online offer
Ends: 31-Dec-2026
655,000
할인액 98,000 (15%)
Each

The LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer.

  • Stable—dyes are freeze dried in separate vials to maintain stability
  • Robust—staining pattern is the same before and after fixation
  • Low compensation—minimal spectral overlap between other fluorophores

Stable

The product is sold in tubes with DMSO and in lyophilized flow tubes and plates which require no DMSO. Amine reactive dyes in solution will lose their effectiveness over a short period of time, therefore it is recommended to completely use material once rehydrated.

Robust

Dead cell discriminator stains can lose sensitivity after treatment with fixatives such as formaldehyde or ethanol-based fixation methods required for intracellular phosphorylation studies. The LIVE/DEAD™ Fixable Violet Stain is an amine reactive dye that binds covalently to intracellular and extracellular amines, and the staining pattern is preserved following formaldehyde fixation.

Low compensation

The LIVE/DEAD™ Fixable Violet Stain was selected based on its fluorescent properties to minimize compensation between other violet dyes and dyes that excite off of the 488 nm blue laser. The violet-fluorescent reactive dye has an excitation maximum of ∼416 nm and is excited well with the 405 nm violet laser, and it has an emission maxima of ∼451 nm, so it can be collected in the first channel on most violet laser flow cytometers.

How it works

In cells with compromised membranes, the dye reacts with free amines both in the cell interior and on the cell surface, yielding intense fluorescent staining. In viable cells, the dye's reactivity is restricted to the cell-surface amines, resulting in less intense fluorescence. The difference in intensity is typically greater than 50-fold between live and dead cells, allowing for easy discrimination.

Colors available

LIVE/DEAD™ Fixable Dead Cell Stains are available in a wide variety of colors to meet your multi-color panel needs.

For Research Use Only. Not for use in diagnostic procedures.
사양
세포 투과성Impermeant
세포 유형Eukaryotic Cells
설명LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation
검출 방법Fluorescence
염료 유형LIVE/DEAD™ Fixable Violet Dead Cell Stain
형태Solid
형식Tube(s)
수량400 Assays
배송 조건Room Temperature
색상Violet
방출 파장 범위451
Excitation Wavelength Range416 nm
용도(애플리케이션)Viability Assay
용도 (장비)Flow Cytometer
제품라인LIVE/DEAD
제품 유형Stain
Unit SizeEach
구성 및 보관
Contains 10 vials of LIVE/DEAD™ fixable dead cell stain and 500 μL DMSO.

Store at -20°C.

자주 묻는 질문(FAQ)

How do I prepare dead cell controls for LIVE/DEAD cell viability assays?

There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.

Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which cell viability kits are compatible with fixation?

The LIVE/DEAD Fixable kits for flow cytometry analysis are compatible with fixation. These kits use amine-reactive cell-impermeant dyes that stain the cell surface of live cells and also the cytosol of dead cells-live cells are dim and dead cells are bright. Since the dye is covalently bound to the cells, it will be retained after fixation. Unfortunately, this method does not work well for imaging-based assays, as all cells are stained and it is difficult to distinguish bright dead cells from dim live cells with a microscope. Ethidium monoazide (EMA; Cat No. E1374) is a cell impermeant nucleic acid stain that can be applied to live cultures and stains only dead cells. After incubation and washing away unbound dye, the cells can be exposed to light to photoactivate EMA to crosslink to dead cell DNA. After crosslinking to dead cell DNA, the samples may be fixed and permeabilized. Image-IT DEAD Green Viability Stain (Cat. No. I10291) for imaging and high-content screening (HCS) analysis is a live-cell impermeant DNA binding dye that is compatible with fixation and permeabilization with good retention up to 48 hours. We also have a LIVE/DEAD Reduced Biohazard Cell Viability Kit (Cat. No. L7013) for imaging and flow analysis that contains two DNA binding dyes, SYTO 10 and Dead Red, that are sufficiently retained to be analyzed soon after 4% glutaraldehyde fixation.
Note: In general, DNA-binding dyes and calcein AM are not compatible with fixation, as these dyes are not covalently bound to components of the cell and will thus slowly diffuse out of cells after fixation, gradually staining all cells as dead.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (3)

인용 및 참조 문헌
Abstract
Amine reactive dyes: an effective tool to discriminate live and dead cells in polychromatic flow cytometry.
Authors:Perfetto SP, Chattopadhyay PK, Lamoreaux L, Nguyen R, Ambrozak D, Koup RA, Roederer M,
Journal:J Immunol Methods
PubMed ID:16756987
'Membrane-damaged cells caused by either mechanical trauma or through normal biological processes can produce artifacts in immunophenotyping analysis by flow cytometry. Dead cells can nonspecifically bind monoclonal antibody conjugates, potentially leading to erroneous conclusions, particularly when cell frequencies are low. To date, DNA intercalating dyes (Ethidium monoazaide (EMA), Propidium Iodide, ... More
SYTO probes in the cytometry of tumor cell death.
Authors:Wlodkowic D, Skommer J, Darzynkiewicz Z,
Journal:Cytometry A
PubMed ID:18260152
Apoptosis is a complex and finely controlled cell death process of great relevance in tissue homeostasis and pathogenesis. The majority of classical apoptotic features can be examined by flow as well as image cytometry. Therefore, cytometry has been used as a technology of choice in studies of tumor cell demise. ... More
Impaired wound healing predisposes obese mice to severe influenza virus infection.
Authors:O'Brien KB, Vogel P, Duan S, Govorkova EA, Webby RJ, McCullers JA, Schultz-Cherry S
Journal:J Infect Dis
PubMed ID:22147799
For the first time, obesity appeared as a risk factor for developing severe 2009 pandemic influenza infection. Given the increase in obesity, there is a need to understand the mechanisms underlying poor outcomes in this population. In these studies, we examined the severity of pandemic influenza virus in obese mice ... More