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인용 및 참조 문헌 (14)
Invitrogen™
LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target자세히 알아보기
| 카탈로그 번호 | 수량 |
|---|---|
| L7010 | 1 kit |
카탈로그 번호 L7010
제품 가격(KRW)
477,000
온라인 행사
Ends: 31-Dec-2025
561,000할인액 84,000 (15%)
Each
수량:
1 kit
제품 가격(KRW)
477,000
온라인 행사
Ends: 31-Dec-2025
561,000할인액 84,000 (15%)
Each
The LIVE/DEAD® Cell-Mediated Cytotoxicity Kit measures natural killer (NK) cell-mediated, lymphokine-activated killer (LAK) cell-mediated and T cell mediated cytotoxicity. Target cells are preincubated with the green-fluorescent membrane stain DiOC18 and then mixed with effector cells in the presence of the red-fluorescent, membrane-impermeant dye, propidium iodide. Live and dead target cells retain the green-fluorescent membrane stain; target and effector cells with compromised membranes exhibit red-fluorescent nucleic acid staining; live effector cells are nonfluorescent.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells, Eukaryotic Cells
설명LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
형식Tube(s), Slide(s)
수량1 kit
배송 조건Room Temperature
색상Green, Red
Emission501/617
Excitation Wavelength Range484, 536 nm
용도(애플리케이션)Viability Assay
용도 (장비)Fluorescence Microscope, Flow Cytometer
제품라인LIVE/DEAD
제품 유형Cell-Mediated Cytotoxicity Kit
Unit SizeEach
구성 및 보관
Store in refrigerator at 2°C to 8°C and protect from light.
자주 묻는 질문(FAQ)
Which optical filter sets are compatible with the LIVE/DEAD Cell-Mediated Cytotoxicity Kit?
인용 및 참조 문헌 (14)
인용 및 참조 문헌
Abstract
Evaluation of human mast cell-mediated cytotoxicity by DIOC18 target cell labeling in flow cytometry.
Journal:J Immunol Methods
PubMed ID:17188705
'(51)Cr release assay (CRA) is still the standard method to study mast cell (MC)-mediated cytotoxicity in vitro. Non-radioactive methods e.g. MTT, Hoechst 22147 staining, have also been used. Though CRA has the benefit of being reproducible, it has several drawbacks e.g. spontaneous release and radioactivity. The basic strategy of this
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells.
Journal:Respir Res
PubMed ID:15857506
'Lung fluid balance in the healthy lung is dependent upon finely regulated vectorial transport of ions across the alveolar epithelium. Classically, the cellular locus of the major ion transport processes has been widely accepted to be the alveolar type II cell. Although evidence is now emerging to suggest that the
Direct visualisation and quantification of cellular cytotoxicity using two colour flourescence.
Journal:J Immunol Methods
PubMed ID:1431162
A fluorescence method is described for the evaluation of cell death induced by cellular cytolytic activity. A green fluorescent membrane dye, D275, was used to label various target cell lines and propidium iodide (PI) uptake was used to assay cell death. Natural killer (NK), lymphokine activated killer (LAK) as well
Rapid flow cytometric assay for the assessment of natural killer cell activity.
Journal:J Immunol Methods
PubMed ID:8228287
A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably separating
Human Vγ2Vδ2 T cells limit breast cancer growth by modulating cell survival-, apoptosis-related molecules and microenvironment in tumors.
Journal:Int J Cancer
PubMed ID:23595559