LIVE/DEAD Sperm Viability Kit는 형광 현미경분석 또는 flow cytometry로 정자의 생존율과 수정력을 분석하는 새로운 형광 기반 검사를 제공합니다. 막 투과성자세히 알아보기
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L7011
1 kit
카탈로그 번호 L7011
제품 가격(KRW)
779,000
Online offer
Ends: 31-Dec-2025
916,000
할인액 137,000 (15%)
Each
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수량:
1 kit
제품 가격(KRW)
779,000
Online offer
Ends: 31-Dec-2025
916,000
할인액 137,000 (15%)
Each
카트에 추가하기
LIVE/DEAD Sperm Viability Kit는 형광 현미경분석 또는 flow cytometry로 정자의 생존율과 수정력을 분석하는 새로운 형광 기반 검사를 제공합니다. 막 투과성 SYBR™ 14 핵산 균주가 살아있는 정자를 녹색 형광으로 표지하고 막 비투과성 propidium iodide가 적색 형광으로 막손상 정자의 핵산을 표지합니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Eukaryotic Cells, Sperm Cells
설명LIVE/DEAD™ Sperm Viability Kit
검출 방법Fluorescence
염료 유형Other Label(s) or Dye(s)
형식Tube(s), Slide(s)
수량1 kit
배송 조건Room Temperature
색상Green, Red
Emission516/617
용도(애플리케이션)Viability Assay
용도 (장비)Fluorescence Microscope, Flow Cytometer
제품라인LIVE/DEAD
제품 유형Sperm Viability Kit
Unit SizeEach
구성 및 보관
Store in freezer at -5°C to -30°C and protect from light.
자주 묻는 질문(FAQ)
What is the fluorescence emission maxima of SYBR 14 dye and propidium iodide?
When bound to DNA, the fluorescence emission maxima of these dyes are 516 nm and 617 nm, respectively. The spectra are available in Figure 1 of the Product Manual.
How do I prepare dead cell controls for LIVE/DEAD cell viability assays?
There are two easy options. One is to heat-inactivate the cells by placing at 60 degrees C for 20 minutes. The second is to subject the cells to 70% ethanol. Alcohol-fixed cells can be stored indefinitely in the freezer until use, potentially up to several years.
Centrifuge cells, pellet, and remove supernatant.
Fix cells: Add 10 mL ice cold 70% ETOH to a 15 mL tube containing the cell pellet, adding dropwise at first while vortexing, mix well.
Store in freezer until use.
When ready to use, wash twice and resuspend in buffer of choice.
Dilution of semen to low cell numbers/dose can result in a bull-dependent reduction in the post-thaw viability of cryopreserved bovine spermatozoa. It is possible that essential seminal plasma components are lacking at the greater dilution rates, thereby contributing to the deleterious effects of semen dilution. Ejaculates of 6 Holstein bulls ... More
Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation.
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) molecular markers linked to genes controlling semen freezability can be identified using amplified restriction fragment length polymorphism (AFLP) technology. Five ejaculates were collected from each of 129 boars. Semen ... More
Comparison of assessment of fowl sperm viability by eosin-nigrosin and dual fluorescence (SYBR-14/PI).
Authors:Chalah T, Brillard JP
Journal:Theriogenology
PubMed ID:10732141
'The kinetics of fowl sperm viability/mortality following short-term and long-term in vitro storage were studied using 2 different staining methods: eosin/nigrosin (observed under light microscopy) and SYBR-14/PI (dual fluorescence). Based on data obtained at 0, 30 min and at 2, 4 and 24 h (T0, T30, T2, T4, and T24) ... More
Seasonal changes of semen quality and freezability in Franches-Montagnes stallions.
Authors:Janett F, Thun R, Bettschen S, Burger D, Hassig M
Journal:Anim Reprod Sci
PubMed ID:12695055
'The objective of this study was to investigate seasonal changes of semen quality parameters in Franches-Montagnes stallions and to compare the freezability of ejaculates collected in autumn and winter. Experiments were performed using 15 stallions from the National Stud Farm in Avenches (Switzerland). Ejaculates were collected and evaluated every month ... More
Flow cytometric assessment of allopurinol susceptibility in Leishmania infantum promastigote.
Authors:Kamau SW, Hurtado M, Müller-Doblies UU, Grimm F, Nunez R
Journal:Cytometry
PubMed ID:10918286
'BACKGROUND: Leishmaniasis is a major tropical and subtropical parasitic disease. Sodium stibogluconate, N-methyl -D-glucamine antimoniate, amphotericin B, pentamidine, and ketoconazole are drugs used to treat this disease. Some of these drugs cause severe adverse side effects and treatment failures are common. Allopurinol, a purine analog, has been used to treat ... More