SilverXpress™ Silver Staining Kit

인용 및 참조 문헌 (1)

Invitrogen™

SilverXpress™ Silver Staining Kit

The SilverXpress Silver Staining Kit is a highly sensitive silver staining kit providing nanogram-level sensitivity with minimal background. The easy-to-follow자세히 알아보기

카탈로그 번호	수량
LC6100	1 kit

카탈로그 번호 LC6100

제품 가격(KRW)

548,000

카트에 추가하기

1 kit

대량 주문 또는 맞춤형 요청

제품 가격(KRW)

548,000

카트에 추가하기

The SilverXpress Silver Staining Kit is a highly sensitive silver staining kit providing nanogram-level sensitivity with minimal background. The easy-to-follow protocol requires less total time than standard Coomassie staining and is complete in a little over one hour.

Compare all silver stains ›

The SilverXpress Silver Staining Kit uses ammoniacal silver chemistry and glutaraldehyde sensitization to produce a highly sensitive silver stain, capable of detecting much lower levels of protein than standard Coomassie or Colloidal Blue techniques. Clear background makes sample identification unambiguous and provides publication-quality gels.

DNA staining
The SilverXpress kit may also be used for detecting nanogram concentrations of DNA that cannot be detected using ethidium bromide. It is five-times more sensitive than ethidium bromide, detecting as little as less than 0.5 ng of 50 bp DNA.

For Research Use Only. Not for use in diagnostic procedures.

검출 위치In-Gel Detection

검출 방법Colorimetric

제품라인SilverXpress

제품 유형Protein Gel Staining Kit

수량1 kit

유통 기한6 Months

배송 조건Room Temperature

타겟 분자Protein

라벨 또는 염료SilverXpress Silver Stain

Unit SizeEach

구성 및 보관

This kit contains sufficient reagents to stain 25 minigels

Contains:
125 mL Sensitizer
125 mL Stainer A
125 mL Stainer B
125 mL Developer
125 Stopper solution

Store kit at 2°C to 8°C. Stable for 6 months when stored under these conditions.

자주 묻는 질문(FAQ)

Can I stain my TBE gel? How?

Yes, you can stain your TBE gels with ethidium bromide, SYBR Green I, SYBR Green II, and the SilverXpress Silver Staining Kit. For ethidium bromide staining, soak the gel in a 2 µg/mL solution of ethidium bromide in ultrapure water for 20 minutes. Destain by rinsing with three successive 10-minute rinses of ultrapure water. Visualize bands under UV light.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverXpress Silver Staining kit and my stained gels are too dark. What went wrong?

Here are possible causes and solutions:

- Stopper not added to the gel at the appropriate time. Be sure to add the stopper slightly before the desired stain intensity is reached.
- Protein is overloaded. Decrease the protein load on the gel.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverXpress Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all SilverXpress™ Silver Staining Kit FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌

Abstract

DNA binding site selection of dimeric and tetrameric Stat5 proteins reveals a large repertoire of divergent tetrameric Stat5a binding sites.

Authors:Soldaini E, John S, Moro S, Bollenbacher J, Schindler U, Leonard WJ

Journal:Mol Cell Biol

PubMed ID:10594041

We have defined the optimal binding sites for Stat5a and Stat5b homodimers and found that they share similar core TTC(T/C)N(G/A)GAA interferon gamma-activated sequence (GAS) motifs. Stat5a tetramers can bind to tandemly linked GAS motifs, but the binding site selection revealed that tetrameric binding also can be seen with a wide ... More