EpiLife™ Medium, with 60 μM calcium
EpiLife™ Medium, with 60 μM calcium
Gibco™

EpiLife™ Medium, with 60 μM calcium

EpiLife is a sterile, liquid medium prepared with 60 μM calcium chloride for the long-term, serum-free culture of human epidermal자세히 알아보기
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카탈로그 번호수량
MEPI500CA500 mL
카탈로그 번호 MEPI500CA
제품 가격(KRW)
158,000
キャンペーン価格
Ends: 31-Dec-2025
175,000
할인액 17,000 (10%)
Each
카트에 추가하기
수량:
500 mL
제품 가격(KRW)
158,000
キャンペーン価格
Ends: 31-Dec-2025
175,000
할인액 17,000 (10%)
Each
카트에 추가하기
EpiLife is a sterile, liquid medium prepared with 60 μM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of an appropriate growth supplement prior to use. EpiLife Medium greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. This basal media is animal origin-free and contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. EpiLife Medium is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2 and 95% air.

To support plating and long term proliferation of human keratinocytes, EpiLife Medium must be supplemented with selected hormones and growth factors. We offer a choice of growth supplements for culturing human keratinocytes. Please refer to the EpiLife Medium user guide to determine the best supplement choice for your purposes. The supplements contain all of the growth factors, hormones, and extracts necessary for growth of human keratinocytes in EpiLife Medium.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Keratinocyte Cells, Epithelial Cells
무기 염류Calcium
제품라인Cascade Biologics, EpiLife
제품 유형EpiLife Medium
수량500 mL
배송 조건Room Temperature
분류Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Culture TypeMammalian Cell Culture
형태Liquid
Serum LevelSerum-free
멸균Sterile-filtered
Sterilization MethodSterile-filtered
첨가제 포함HEPES, Phenol Red
Unit SizeEach
구성 및 보관
Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.