Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes
Invitrogen™

Neon™ NxT Electroporation System 10-μL Kit with 1-Channel Tubes

The Neon NxT Electroporation System 10-μL Kit is designed specifically for use with the Neon NxT Electroporation System.
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카탈로그 번호반응 수
N102525 x 2 Reactions
N109696 x 2 Reactions
카탈로그 번호 N1025
제품 가격(KRW)
868,000
Each
카트에 추가하기
반응 수:
25 x 2 Reactions
제품 가격(KRW)
868,000
Each
카트에 추가하기

The Neon NxT Electroporation System 10-μL Kit with 1-Channel Tubes is designed specifically for use with the Neon NxT Electroporation System (Cat. Nos. NEON1S, NEON1SK, NEON18S, NEON18SK). Use this kit for transfection volumes of 10 μL, containing 5 x 104–2 x 105 adherent cells or 1 x 105–5 x 105 suspension cells. Cells that have been transfected using the included 10-μL Neon NxT tips are ready to be washed and plated into a multi-well culture dish.

For Research Use Only. Not for use in diagnostic procedures.
사양
용도(애플리케이션)Transfection
용도(장비)Neon™ NxT Electroporation System
반응 수25 x 2 Reactions
제품 유형Pipette Tip
수량25 x 2 reactions
부피(미터법) 샘플10 μL
셀 유형Stem Cells, Primary Cells, Hard-to-Transfect Cells
형식Electroporation Pipette Tip
Transfection TechniqueElectroporation
Unit SizeEach
구성 및 보관
• 1 mL Resuspension R Buffer; store at 4°C
• 1 mL Resuspension T Buffer; store at 4°C
• 1 mL Resuspension Genome Editing Buffer; store at 4°C
• 50 mL Electrolytic E10 Buffer; store at 4°C
• 25 reaction tips
• 8 electroporation 1-channel tubes

자주 묻는 질문(FAQ)

Should I warm Neon NxT buffers before use?

We recommend warming the buffers to room temperature before use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I aliquot and store Neon NxT buffers?

We recommend storing Neon NxT buffers in the original containers at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What temperature should Neon NxT buffers be stored at?

Neon NxT buffers should be stored at 4 degrees C. Temperature fluctuations and storage at room temperature have been shown to increase the chances for precipitate formation. Therefore, the desired amount of buffer for an experiment should be removed from the buffer bottle and the bottle should be immediately returned to 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What state should the cells be in for best results with the Neon Nxt Electoporation System?

For best results with the Neon Nxt Electoporation System, the cells should be healthy (not stressed) and actively dividing so that the nuclear membrane integrity is already weakened for payload needing to enter the nucleus. Consider “starving” the cells of serum or other essential nutrients.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

I'm experiencing arcing issues with the Neon Nxt Electoporation System. What could be causing this?

Arcing could be caused by high voltage or pulse length settings, high salt or contaminants in the DNA sample, incorrect cell density, and air bubbles formed during mixing of cell samples. Mix samples gently to avoid air bubble formation and pipette samples in a slow, smooth motion to avoid air uptake.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.