NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each
인용 및 참조 문헌 (1)
Invitrogen™
NeuroTrace™ Multicolor Tissue-Labeling Kit - DiO, DiI, DiD pastes, 500 mg each
NeuroTrace™ Multicolor Tissue-Labeling Kit contains 500 mg each of DiI, DiO or DiD mixed into an inert, water-resistant gel. The자세히 알아보기
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카탈로그 번호
수량
N22884
1 kit
카탈로그 번호 N22884
제품 가격(KRW)
758,000
Online offer
Ends: 31-Dec-2025
891,000
할인액 133,000 (15%)
Each
카트에 추가하기
수량:
1 kit
제품 가격(KRW)
758,000
Online offer
Ends: 31-Dec-2025
891,000
할인액 133,000 (15%)
Each
카트에 추가하기
NeuroTrace™ Multicolor Tissue-Labeling Kit contains 500 mg each of DiI, DiO or DiD mixed into an inert, water-resistant gel. The paste is ready to use as supplied and can be applied directly to live or fixed tissue specimens using the tip of a needle. This method of application improves the penetration of the dye into bundled neurons, labeling axons both on and below the surface.
For Research Use Only. Not for use in diagnostic procedures.
사양
색상Green, Yellow, Red
검출 방법Fluorescence
용도(장비)Fluorescence Microscope
제품 유형Tissue Labeling Kit
수량1 kit
배송 조건Room Temperature
세포하위구조 국지화Cell Membranes & Lipids
제품라인NEUROTRACE
Unit SizeEach
구성 및 보관
Store at room temperature and protect from light.
자주 묻는 질문(FAQ)
I am doing retrograde neuronal labeling using the NeuroTrace DiI Paste. I apply a small glob onto the neuron of interest, but it is not adhering very well. What can I do?
This paste, which has DiI C18 uniformly dispersed within a proprietary hydrophobic carrier, is commonly used to dab onto larger areas than smaller areas commonly used with crystals. Any moisture on the area the paste is applied may be the cause for lack of adherence. Apply the paste using a small flat-head spatula or toothpick and keep it in place a bit longer to ensure contact. The area may also be covered with parafilm to ensure contact as well.
How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?
The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.
Which form of the lipophilic tracers (DiO, DiI, DiD, etc) should I use?
Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.
Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.