Rat Primary Cortical Astrocytes
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Rat Primary Cortical Astrocytes
Gibco™

Rat Primary Cortical Astrocytes

Gibco Rat Primary Cortical Astrocytes provide neuroscience researchers with a ready-to-use, highly pure source of astrocytes for experiments.
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카탈로그 번호수량
N7745100
N7745-100으로도 사용됨
1 x 106 cells
카탈로그 번호 N7745100
N7745-100으로도 사용됨
제품 가격(KRW)
999,000
Each
카트에 추가하기
수량:
1 x 106 cells
제품 가격(KRW)
999,000
Each
카트에 추가하기

Gibco Rat Primary Cortical Astrocytes are isolated and cryopreserved from the cortex of Sprague Dawley rats at day E19 of gestation. The astrocytes are passaged only once before cryopreservation and consistently yield post-thaw viability of 70% or greater. Each vial contains 1 million cells per mL and provides a flexible, ready-to-use, and quality alternative to fresh astrocytes.

Rat Primary Cortical Astrocyte details

  • Exhibit ≥70% viability upon thawing
  • Stain >80% positive for the astrocyte specific marker glial fibrillary acid protein (GFAP)
  • Stain ≤10% positive for neuron and oligodendrocyte specific markers galactocerebroside (GalC) and doublecortin (DCX) or tubulin beta-3 (TUBB3)
  • Exhibit a doubling time of approximately nine days at P2
  • Can be expanded in culture for at least one passage
For Research Use Only. Not for use in diagnostic procedures.
사양
셀 수1 x 106 cells
제품 유형Rat Cortical Astrocyte
수량1 x 106 cells
테스트됨Cell Purity, Sterility, Viability, Mycoplasma
AgeFetal
셀 유형Glial Cells
Culture TypeMammalian Cell Culture
형태Cryopreserved
Rat
TissueCortex
Unit SizeEach
구성 및 보관
Store in liquid nitrogen, vapor-phase.

자주 묻는 질문(FAQ)

What are some of the key applications for astrocytes?

Astrocytes can be used to research cell transplantation, intracellular transport, ion-channel function, toxicity, receptor signaling, analyses of electrophysiological properties, neurotransmitter release, and evaluation of interaction between neurons and astrocytes.

I used rat primary cortical astrocytes from another company and observed cell aggregates after thawing but not with the astrocytes from your company. Why?

Gibco Rat Primary Cortical Astrocytes were dissociated into single cells before cryopreservation, which gives an advantage to control the number of cells to plate.

How can I coat Gibco Rat Primary Cortical Astrocytes on glass coverslips?

We recommend cleaning the coverslips and then coating them with 0.01% poly-D-lysine in double distilled water.

What is the doubling time of Gibco Rat Primary Cortical Astrocytes?

The doubling time of Gibco Rat Primary Cortical Astrocytes is about 8.7 days.

For how many passages can I expand Gibco Rat Primary Cortical Astrocytes?

Gibco Rat Primary Cortical Astrocytes can be thawed, recovered, and proliferated up to three passages to yield 1.5-2 fold increase of cells.