PathoProof™ Complete-16 Kits for Applied Biosystems™ 7500 and 7500 Fast qPCR systems

Good-quality raw milk must meet accepted standards for chemical composition and purity as well as levels of a variety of mastitis-causing microorganisms. Identify 15 of the main mastitis-causing microorganisms and the Staphylococcal β-lactamase gene responsible for the penicillin resistance in Staphylococci within four hours using PathoProof Complete-16 multiplex PCR. All targeted species are detected simultaneously, overcoming the common problem of mixed growth results in bacterial culturing.

• Ensure milk and milk products are safe and suitable for their intended uses
• Remove affected cows from the herd to ensure optimal milk quality
• Enables faster decisions on treatment
• Reduces time available for infective agents to spread to healthy herd mates
• Reduces broad spectrum antibiotic treatment
• Rapid treatment shortens inflammation time

Assays are based on real-time PCR, an accurate and sensitive technology for identification of the target DNA in the sample. Tests can be used for analyzing fresh and preserved quarter milk samples, milk recording samples, and bulk tank milk samples.

Achieve high sensitivity, even for samples that show no growth in culture. Accurate detection of viable, dead, and growth-inhibited microorganisms reduces no-growth results.

PathoProof Complete-16 Kit identifies:

1. Staphylococcus aureus
2. Staphylococcus spp. (including all major coagulase-negative staphylococci)
3. Streptococcus agalactiae
4. Streptococcus dysgalactiae
5. Streptococcus uberis
6. Escherichia coli
7. Enterococcus spp. (including E. faecalis and E. faecium)
8. Klebsiella oxytoca and K. pneumoniae
9. Serratia marcescens
10. Corynebacterium bovis
11. Arcanobacter pyogenes and Peptoniphilus (Peptostreptococcus) indolicus
12. Staphylococcal β-lactamase gene (penicillin-resistance gene)
13. Mycoplasma bovis
14. Mycoplasma spp.
15. Yeast
16. Prototheca spp

Automated sample preparation
Significantly reduce the hands-on time needed when processing larger numbers of samples with the semi-automated Thermo Scientific™ KingFisher™ Flex instrument for magnetic bead-based DNA purification and extraction, along with PathoProof Mastitis Complete-16 KingFisher Kits and qPCR reagents.

Not all products are available for sale in all territories. Please inquire.