Gibco™

BMPR1A Recombinant Human Protein

Bone Morphogenetic Protein Receptor 1A (BMPR1A) Fc Chimera is a bioactive protein intended for use in cell culture applications. BMPR1A자세히 알아보기

카탈로그 번호	수량
PHC9245	25 μg

카탈로그 번호 PHC9245

제품 가격(KRW)

25 μg

Bone Morphogenetic Protein Receptor 1A (BMPR1A) Fc Chimera is a bioactive protein intended for use in cell culture applications. BMPR1A is one of the bone morphogenetic protein (BMP) receptors which are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

• High purity—no interference from other proteins or contaminants
• High biological activity—more results with less protein
• Proven compatibility—Gibco™ proteins bioassayed with Gibco™ media

High Purity Recombinant Bioactive Protein
Bone Morphogenetic Protein Receptor 1A Fc Chimera is one of our more than 250 available Gibco™ recombinant proteins. To help ensure Gibco™ growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco™ Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Bone Morphogenetic Protein Receptor 1A (BMPR1A) specific activity is determined by its ability to inhibit human BMP4induction of alkaline phosphatase in the mouse chondrogenic cell line ATDC5.

Proven Compatibility
Gibco™ proteins are bioassayed with Gibco™ media. Since 1962, Gibco™ has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco™ growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

등록 번호NM_004329

생물학적 활성ED₅₀ range = 0.1 to 0.3 μg/ml

분류Carrier-Free

엔도톡신 수준< 0.1 ng/μg

발현 시스템Mammalian

유전자 별칭BMPR1A

유전자 ID(Entrez)BMPR1a

인디케이터 셀mouse chondrogenic cell line ATDC5

질량25 μg

분자량43.7 kDa

제품라인Gibco

단백질군Growth Factors & Receptors

단백질 형태Recombinant, Ligand, Fc Chimera

단백질 아형BMPs (Bone Morphogenetic Proteins) & Related Factors

단백질 태그Untagged

정제 방법Sequential Chromatography

순도 또는 품질 등급95 %

수량25 μg

반응성Human

연구 카테고리Stem Cell Research, Neurobiology

배송 조건Wet Ice

특이 활성ED50 range = 0.1 to 0.3 µg/ml

용도(애플리케이션)Cell Culture

형태Lyophilized

재조합Recombinant

종Human

Unit Size25 µg

구성 및 보관

Store lyophilized human BMPR1a at 2 to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤ –20°C. Avoid repeated freeze⁄thaw cycles.

자주 묻는 질문(FAQ)

It looks like the recombinant protein vial I received from you is empty. Is this normal?

Gibco recombinant proteins are frequently formulated without carrier proteins or additives (e.g., BSA, HSA, sucrose, etc.) and no Gibco PeproTech recombinant protein contains a carrier protein. As a result, during lyophilization, the protein product may be deposited on the vial as a thin, and sometimes invisible, film instead of a pellet. The size of the pellet, if any, is not directly related to the quantity of the recombinant protein in the vial. Our quality control procedures assure that each vial contains the correct amount of product.

To ensure complete recovery of protein product, before opening a vial of lyophilized recombinant protein, we recommend centrifuging it in a microcentrifuge for 20-30 seconds to drive any protein that may be lodged in the cap or on the side to the bottom of the vial. After reconstitution, you can confirm the presence of product protein by running a small amount on SDS-PAGE. In general, a protein band with expected size should be visible with as little as 10 ng of protein loaded on an acrylamide gel.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does my recombinant protein not show activity in my own experiment?

Assay time is critical. Each assay needs to beoptimized and performed at the peak response time. Different cells may respond differently to a growth factor or cytokine. We suggest repeating our QC assay using same indicator cells as suggested in the manual to see if you can obtain a similar response. In addition, serum may be masking the response. Serum starvation might be needed for certain types of assays.

How should I store the reconstituted recombinant proteins?

Protein solutions are generally not very stable when frozen at low concentration. Upon freeze and thaw, some proteins in the solution may stick to the wall of the container, which results in significant reduction of protein concentration if the starting concentration was low. Therefore, carrier proteins are used to reduce such loss. The most commonly used carrier proteins include bovine serum albumin (BSA), human serum albumin (HSA), or fetal bovine serum (FBS). These carrier proteins are generally used at 0.1% concentration. As a rule of thumb, if the concentration of the recombinant protein is less than 0.5 mg/mL, it is a good idea to add some carrier protein

How do I store my lyophilized recombinant protein?

Lyophilized proteins can typically be stored at 2 to 8 degrees C for several weeks, or stored dessicated at -20 degrees C for long-term storage.