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인용 및 참조 문헌 (1)
Fluorescein-Ubiquitin
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to자세히 알아보기
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보기 방식 변경
| 카탈로그 번호 | 수량 |
|---|---|
| PV4378 | 500 μg |
| PV4377 | 50 μg |
카탈로그 번호 PV4378
제품 가격(KRW)
-
수량:
500 μg
Fluorescein-labeled ubiquitin for use as a general research tool or in high throughput assays to monitor protein ubiquitination. Due to the method in labeling the ubiquitin protein, all of the lysines with the protein are available for further conjugation to form poly-ubiquitin chains.
For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For use in combination with FRET donors including LanthaScreen™ Tb-Ubiquitin (PV4375) or a LanthaScreen™ antibody including Tb-anti-GST (PV4216) or Tb-anti-ubiquitin-FK2 (PV4751).
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
여기/방출340⁄490, 520
용도(장비)Microplate Reader
라벨 유형Classic Dyes
라벨 또는 염료FITC (Fluorescein)
수량500 μg
판독End Point
배송 조건Dry Ice
기질 특성Protein-Based Substrate
기술TR-FRET
콘주게이트Fluorescein
형태Liquid
Unit SizeEach
구성 및 보관
Fluorescein-labeled ubiquitin in HEPES buffered saline. Store at -20°C.
인용 및 참조 문헌 (1)
인용 및 참조 문헌
Abstract
TR-FRET-based high-throughput screening assay for identification of UBC13 inhibitors.
Journal:J Biomol Screen
PubMed ID:22034497
UBC13 is a noncanonical ubiquitin conjugating enzyme (E2) that has been implicated in a variety of cellular signaling processes due to its ability to catalyze formation of lysine 63-linked polyubiquitin chains on various substrates. In particular, UBC13 is required for signaling by a variety of receptors important in immune regulation,