The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.To use, simply add the BacMam enhancer to자세히 알아보기
Have Questions?
카탈로그 번호
수량
PV5835
150 μL
카탈로그 번호 PV5835
제품 가격(KRW)
143,000
Each
수량:
150 μL
제품 가격(KRW)
143,000
Each
The BacMam Enhancer helps to increase expression of BacMam reagents in mammalian cells.
To use, simply add the BacMam enhancer to cell culture media with or without serum that have already been treated with the BacMam reagent, then incubate cells and replace with fresh medium. Different cell types vary in their response to the BacMam enhancer and for best results the amount and treatment time will need to be optimized.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Mammalian Cells
농도1000 X
설명Histone Deacetylase Inhibitor
용도(애플리케이션)BacMam-Enabled Cellular Assays
수량150 μL
배송 조건Dry Ice
형태Frozen
제품 유형BacMam Enhancer Solution
Unit SizeEach
구성 및 보관
Concentration: 1.5 mM (1000X) Store at -20°C.
자주 묻는 질문(FAQ)
I am using your CellLight BacMam reagents for fluorescent protein-labeling of my cell organelles, but they aren't labeling well. How can I improve it?
First, make sure that your cells are mammalian and non-blood derived. If the cells have low transduction efficiency (i.e., only a few cells are labeling or are labeling only dimly), first try increasing the particle number per cell. The next thing to try is to use the BacMam Enhancer Solution. If, however, your cells show labeling, but the localization seems to be too generalized or ubiquitous within the cell, then you should reduce the particle number per cell. The manual gives a guideline and formula for calculating particle number per cell.