Qdot™ 605 ITK™ Carboxyl Quantum Dots
Qdot™ 605 ITK™ Carboxyl Quantum Dots
인용 및 참조 문헌 (14)
Invitrogen™

Qdot™ 605 ITK™ Carboxyl Quantum Dots

Qdot™ 605 ITK™ carboxyl quantum dot는 biomolecule 부하량이 높아야 하는 맞춤형 conjugate 조제에 이상적인 시작 물질입니다. 이들 물질은 carboxylate 기능을자세히 알아보기
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카탈로그 번호수량
Q21301MP250 μL
카탈로그 번호 Q21301MP
제품 가격(KRW)
747,000
Online offer
Ends: 31-Dec-2025
878,000
할인액 131,000 (15%)
Each
카트에 추가하기
수량:
250 μL
제품 가격(KRW)
747,000
Online offer
Ends: 31-Dec-2025
878,000
할인액 131,000 (15%)
Each
카트에 추가하기
Qdot™ 605 ITK™ carboxyl quantum dot는 biomolecule 부하량이 높아야 하는 맞춤형 conjugate 조제에 이상적인 시작 물질입니다. 이들 물질은 carboxylate 기능을 가지며 EDC-매개 응축을 사용해 단백질의 아민군과 변형 oligonucleotide에 결합할 수 있습니다. 이들 probe의 코팅은 본사 Qdot™ ITK™ amino quantum dot 보다 결합 부위가 많지만 비특이 상호작용을 방지하는 PEG linker가 부족합니다. 이들 물질을 X-PEG-amine bi-functional linker에 접합하여 반응을 맞춤화하고 특이성을 높일 수 있습니다. 본사의 Qdot™ ITK™ carboxyl quantum dots는 8 μM 용액으로 제공되며 Qdot™ 프로브 색상 9개를 모두 이용할 수 있습니다.

Qdot™ ITK™ Carboxyl Quantum Dot의 주요 특징:

• Qdot™ 605 ITK™ carboxyl quantum dot 최대 방출: 605 nm
• 높은 광안정성, 밝은 형광
• 단일 라인 여기원으로 효율적으로 방출
• 좁은 방출 및 넓은 스크로크 변화
• 여러가지 색상으로 구매 가능
• 다양한 라벨링 및 추적 어플리케이션에 이상적

Qdot™ Nanocrystal의 특징
Qdot™ probe는 밝은 형광 신호 및/또는 실시간 추적이 필요한 어플리케이션에 이상적입니다. 형광 시약 중 유일하게 Qdot™ probe의 9가지 색상은 모두 단일 (UV --> 청색-녹색) 광원으로 동시에 여기할 수 있습니다. 이 특성은 사용자가 편리하게 사용할 수 있는 경제적인 multiplexing 어플리케이션에 매우 적합한 시약입니다. Qdot™ label은 반도체 나노기술에 기반하며 중간 크기의 단백질과 크기가 유사합니다.

Innovator’s Tool Kit Qdot™ ITK™ Reagent 설명
Qdot™ ITK™ probe는 해당 어플리케이션에 특이적인(non-stocked) conjugate을 준비하거나 맞춤화가 가능한 conjugation 기능이 필요한 연구자들에게 이상적입니다.

다른 유형의 Qdot™ Nanocrystal을 이용할 수 있습니다.
carboxyl-derivatized 유형 외에 본사는 amino와 aliphatic hydrocarbon이 변형된 Qdot™ ITK™ quantum dot도 제공합니다. 본사는 다양한 Qdot™ nanocrystals conjugate와 라벨링 키트도 개발하였습니다. 자세한 정보는 Qdot™ nanocrystals 속성을 조사하거나 Molecular Probes™ 지침서 6.6항—Qdot™ Nanocrystals을 참조하십시오.

본 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 반응성Amine
농도8 μM
방출605
라벨 또는 염료Qdot™ 605
제품 유형Quantum Dot
수량250 μL
반응성 부분Carboxylic Acid
배송 조건Room Temperature
라벨 유형Qdot Nanocrystals
제품라인ITK, Qdot
Unit SizeEach
구성 및 보관
Store in refrigerator (2–8°C).

자주 묻는 질문(FAQ)

How large are the Qdot nanocrystals?

The core/shells are only a few nanometers in diameter (some are elliptical), but with the outer polymer coatings, a fully-functionalized Qdot nanocrystal can range from 15 to 21 nm in hydrodynamic diameter, similar in size to some proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Qdot™ 605 ITK™ Carboxyl Quantum Dots FAQs

인용 및 참조 문헌 (14)

인용 및 참조 문헌
Abstract
Binding of muscimol-conjugated quantum dots to GABAC receptors.
Authors:Gussin HA, Tomlinson ID, Little DM, Warnement MR, Qian H, Rosenthal SJ, Pepperberg DR,
Journal:J Am Chem Soc
PubMed ID:17147380
'Functionalization of highly fluorescent CdSe/ZnS core-shell nanocrystals (quantum dots, qdots) is an emerging technology for labeling cell surface proteins. We have synthesized a conjugate consisting of approximately 150-200 muscimols (a GABA receptor agonist) covalently joined to the qdot via a poly(ethylene glycol) (PEG) linker (approximately 78 ethylene glycol units) and ... More
Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling.
Authors:Krutzik PO, Nolan GP
Journal:Nat Methods
PubMed ID:16628206
'Flow cytometry allows high-content, multiparameter analysis of single cells, making it a promising tool for drug discovery and profiling of intracellular signaling. To add high-throughput capacity to flow cytometry, we developed a cell-based multiplexing technique called fluorescent cell barcoding (FCB). In FCB, each sample is labeled with a different signature, ... More
Water-soluble quantum dots for multiphoton fluorescence imaging in vivo.
Authors:Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW
Journal:Science
PubMed ID:12775841
'The use of semiconductor nanocrystals (quantum dots) as fluorescent labels for multiphoton microscopy enables multicolor imaging in demanding biological environments such as living tissue. We characterized water-soluble cadmium selenide-zinc sulfide quantum dots for multiphoton imaging in live animals. These fluorescent probes have two-photon action cross sections as high as 47,000 ... More
The dynamic control of kiss-and-run and vesicular reuse probed with single nanoparticles.
Authors:Zhang Q, Li Y, Tsien RW,
Journal:Science
PubMed ID:19213879
'Vesicular secretion of neurotransmitter is essential for neuronal communication. Kiss-and-run is a mode of membrane fusion and retrieval without the full collapse of the vesicle into the plasma membrane and de novo regeneration. The importance of kiss-and-run during efficient neurotransmission has remained in doubt. We developed an approach for loading ... More
Targeted tumor cell internalization and imaging of multifunctional quantum dot-conjugated immunoliposomes in vitro and in vivo.
Authors:Weng KC, Noble CO, Papahadjopoulos-Sternberg B, Chen FF, Drummond DC, Kirpotin DB, Wang D, Hom YK, Hann B, Park JW,
Journal:Nano Lett
PubMed ID:18712930
'Targeted drug delivery systems that combine imaging and therapeutic modalities in a single macromolecular construct may offer advantages in the development and application of nanomedicines. To incorporate the unique optical properties of luminescent quantum dots (QDs) into immunoliposomes for cancer diagnosis and treatment, we describe the synthesis, biophysical characterization, tumor ... More
View all Qdot™ 605 ITK™ Carboxyl Quantum Dots citations