Qdot™ 605 ITK™ Amino (PEG) Quantum Dots
Qdot™ 605 ITK™ Amino (PEG) Quantum Dots
인용 및 참조 문헌 (5)
Invitrogen™

Qdot™ 605 ITK™ Amino (PEG) Quantum Dots

Qdot™ 605 ITK™ amino (PEG) quantum dot는 매우 밝고 광안정성이 높은 형광 라벨 단백질과 기타 biopolymer의 맞춤 제조에 이상적인 시작자세히 알아보기
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카탈로그 번호수량
Q21501MP250 μL
카탈로그 번호 Q21501MP
제품 가격(KRW)
1,090,000
Online offer
Ends: 31-Dec-2025
1,282,000
할인액 192,000 (15%)
Each
카트에 추가하기
수량:
250 μL
제품 가격(KRW)
1,090,000
Online offer
Ends: 31-Dec-2025
1,282,000
할인액 192,000 (15%)
Each
카트에 추가하기
Qdot™ 605 ITK™ amino (PEG) quantum dot는 매우 밝고 광안정성이 높은 형광 라벨 단백질과 기타 biopolymer의 맞춤 제조에 이상적인 시작 물질입니다. 이들 probe는 아민이 비활성화된 PEG로 기능하여 비특이적 상호작용를 방지하고 conjugation을 편리하게 취급할 수 있습니다. 이 amino quantum dot는 EDC 등과 같은 수용성 carbodiimide를 사용해 isothiocyanates 및 succinimidyl esters 또는 native carboxylic acid와 효율적으로 반응합니다. 이런 파생물은 초강력 밝기와 형광 안정성을 요하는 다양한 어플리케이션 라벨링과 추적에 사용할 수 있습니다. 자사의 Qdot™ ITK™ amino quantum dot는 8 μM 용액으로 제공되며 8가지 색상 Qdot™ probe로 구매가능합니다.

Qdot™ ITK™ Amino Quantum Dot의 주요 특징:

• Qdot™ 605 ITK™ amino quantum dot 최대 방출: 605 nm
• 높은 광안정성, 밝은 형광
• 단일 라인 여기원으로 효율적으로 방출
• 좁은 방출 및 넓은 스크로크 변화
• 여러가지 색상으로 구매 가능
• 다양한 라벨링 및 추적 어플리케이션에 이상적임

Qdot™ Nanocrystal의 특징
Qdot™ probe는 밝은 형광 신호 및/또는 실시간 추적이 필요한 어플리케이션에 이상적입니다. 형광 시약 중 유일하게 Qdot™ probe의 9가지 색상은 모두 단일 (UV --> 청색-녹색) 광원으로 동시에 여기할 수 있습니다. 이 특성은 사용자가 편리하게 사용할 수 있는 경제적인 multiplexing 어플리케이션에 매우 적합한 시약입니다. Qdot™ label은 반도체 나노기술에 기반하며 중간 크기의 단백질과 크기가 유사합니다.

Innovator’s Tool Kit Qdot™ ITK™ Reagents
이들 Qdot™ ITK™ probe는 어플리케이션에 특이적인(non-stocked) conjugate을 만들거나 맞춤형 conjugation 기능을 원하는 연구자에게 이상적입니다.

여러 형태의 Qdot™ Nanocrystals가 구매가능합니다.
amine-derivatized 제형 외에 carboxyl과 aliphatic hydrocarbon이 조작된 Qdot™ ITK™ quantum dots도 제공합니다. 본사는 다양한 Qdot™ nanocrystals conjugate와 라벨링 키트도 개발하였습니다. 자세한 정보는 Qdot™ nanocrystals 속성을 조사하거나 Molecular Probes™ 지침서 6.6항—Qdot™ Nanocrystals을 참조하십시오.

본 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 반응성Carboxylic Acid, Ketone, Aldehyde
농도8 μM
방출605
라벨 또는 염료Qdot™ 605
제품 유형Quantum Dot
수량250 μL
반응성 부분Amine, Primary Amine
배송 조건Room Temperature
라벨 유형Qdot Nanocrystals
제품라인ITK, Qdot
Unit SizeEach
구성 및 보관
Store in refrigerator (2–8°C).

자주 묻는 질문(FAQ)

How large are the Qdot nanocrystals?

The core/shells are only a few nanometers in diameter (some are elliptical), but with the outer polymer coatings, a fully-functionalized Qdot nanocrystal can range from 15 to 21 nm in hydrodynamic diameter, similar in size to some proteins.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the best way to remove white precipitate from my ITK Qdot nanocrystals?

Spinning your ITK Qdot nanocrystals at approximately 3,000 rpm for 3-5 minutes should remove the white precipitate from the supernatant. Use the supernatant immediately.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I see a white precipitate in my ITK Qdot nanocrystals; should I be concerned?

The precipitate in the organic ITK Qdot nanocrystals occurs with some frequency. The ITK Qdot nanocrystals sometimes include impurities that show as a white precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do my Qdot nanocrystals appear to be blinking?

Blinking is an inherent property of quantum dots; in fact, all single-luminescent molecules blink, including organic dyes. The brightness and photostability of Qdot nanocrystals makes the blinking more visibly apparent. Under higher energy excitation, Qdot nanocrystals blink even faster.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My Qdot nanocrystals were brightly fluorescent before I mounted my samples; now I'm seeing a loss of fluorescence. Why is this happening?

Appropriate mounting media selection is very important to retain the fluorescence of Qdot nanocrystals. In our studies, Qdot nanocrystals work best with the following mountants:

HistoMount medium (Cat No. 00-8030); best for long term archiving
Cytoseal 60 Mountant
Clarion Mountant
Most polyvinyl alcohol-based mountants (limited storage time, less than weeks)
Water-based mountants (limited storage time, less than week)
Up to 50% glycerol (limited storage time, less than week)
Note: We do not recommend using ProLong mounting media with Qdot nanocrystals as it will quench their fluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Qdot™ 605 ITK™ Amino (PEG) Quantum Dots FAQs

인용 및 참조 문헌 (5)

인용 및 참조 문헌
Abstract
Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores.
Authors:Nikiforov TT, Beechem JM
Journal:Anal Biochem
PubMed ID:16860286
'We studied the fluorescence resonance energy transfer (FRET) between quantum dots emitting at 565, 605, and 655 nm as energy donors and Alexa Fluor fluorophores with absorbance maxima at 594, 633, 647, and 680 nm as energy acceptors. As a first step, we prepared covalent conjugates between all three types ... More
Quantum dot targeting with lipoic acid ligase and HaloTag for single-molecule imaging on living cells.
Authors:Liu DS, Phipps WS, Loh KH, Howarth M, Ting AY,
Journal:ACS Nano
PubMed ID:23181687
'We present a methodology for targeting quantum dots to specific proteins on living cells in two steps. In the first step, Escherichia coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13 amino acid recognition sequence that is genetically fused to a protein of interest. In the second ... More
Quantum dots provide an optical signal specific to full collapse fusion of synaptic vesicles.
Authors:Zhang Q, Cao YQ, Tsien RW,
Journal:Proc Natl Acad Sci U S A
PubMed ID:17968015
Synaptic vesicles are responsible for releasing neurotransmitters and are thus essential to brain function. The classical mode of vesicle recycling includes full collapse of the vesicle into the plasma membrane and clathrin-mediated regeneration of a new vesicle. In contrast, a nonclassical mode known as  ... More
Novel system to achieve one-pot modification of cargo molecules with oligoarginine vectors for intracellular delivery.
Authors:Takayama K, Tadokoro A, Pujals S, Nakase I, Giralt E, Futaki S,
Journal:Bioconjug Chem
PubMed ID:19161253
There is a growing number of reports showing the usefulness of cell-penetrating peptides (CPPs) including oligoarginines for intracellular delivery of macromolecules. Although the covalent attachment of the CPP segments to the cargo molecules is usually required to ensure effective delivery, conventional methods of conjugation need several manipulation steps that are ... More
Optical coding of mammalian cells using semiconductor quantum dots.
Authors:Mattheakis LC, Dias JM, Choi YJ, Gong J, Bruchez MP, Liu J, Wang E
Journal:Anal Biochem
PubMed ID:15051536
Cell-based assays are widely used to screen compounds and study complex phenotypes. Few methods exist, however, for multiplexing cellular assays or labeling individual cells in a mixed cell population. We developed a generic encoding method for cells that is based on peptide-mediated delivery of quantum dots (QDs) into live cells. ... More