Quant-iT™ Protein Assay Kit

인용 및 참조 문헌 (6)

Invitrogen™

Quant-iT™ Protein Assay Kit

The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and자세히 알아보기

카탈로그 번호	수량
Q33210	1 Kit

카탈로그 번호 Q33210

제품 가격(KRW)

589,000

Online offer

Ends: 31-Dec-2025

692,000

할인액 103,000 (15%)

카트에 추가하기

1 Kit

대량 주문 또는 맞춤형 요청

제품 가격(KRW)

589,000

Online offer

Ends: 31-Dec-2025

692,000

할인액 103,000 (15%)

카트에 추가하기

The Quant-iT Protein Assay Kit makes protein quantitation easy and accurate. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent, load it into the wells of a microplate, add 1-20 μL of sample, mix, then measure the fluorescence. The assay is highly selective for protein and exhibits very little protein-to-protein variation. The assay is performed at room temperature, and the signal is stable for 3 hours. Common contaminants, such as salts, solvents, or DNA—but not detergents—are well tolerated in the assay. Quant-iT DNA Assay Kits (Q33120, Q33130) and a Quant-iT RNA Assay Kit (Q33140) are also available.

For Research Use Only. Not for use in diagnostic procedures.

어세이Fluorescent Protein Assay

용도(장비)Microplate Reader

유전자 ID(Entrez)3919448

키트 구성품Concentrated assay reagent, dilution buffer, and pre-diluted BSA standards

제품라인Quant-iT

제품 유형Protein Quantitation Assay

수량1 Kit

샘플 부피1 to 20 uL

배송 조건Room Temperature

보관 요구 사항Store in refrigerator (2° to 8°C). Protect from light.

검출 방법Fluorescence

Unit SizeEach

구성 및 보관

Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ Protein Assay Kit FAQs

인용 및 참조 문헌 (6)

인용 및 참조 문헌

Abstract

Acrolein inhibits cytokine gene expression by alkylating cysteine and arginine residues in the NF-kappaB1 DNA binding domain.

Authors:Lambert C, Li J, Jonscher K, Yang TC, Reigan P, Quintana M, Harvey J, Freed BM,

Journal:J Biol Chem

PubMed ID:17491020

Cigarette smoke is a potent inhibitor of pulmonary T cell responses, resulting in decreased immune surveillance and an increased incidence of respiratory tract infections. The alpha,beta-unsaturated aldehydes in cigarette smoke (acrolein and crotonaldehyde) inhibited production of interleukin-2 (IL-2), IL-10, granulocyte-macrophage colony-stimulating factor, interferon-gamma, and tumor necrosis factor-alpha by human T ... More

Functional selection of phagocytosis-promoting genes: cell sorting-based selection.

Authors:Jeon H, Go Y, Seo M, Lee WH, Suk K,

Journal:J Biomol Screen

PubMed ID:20660795

Phagocytosis is a critical host defense mechanism that clears invading pathogens, apoptotic cells, and cell debris; it is an essential process for normal development, tissue remodeling, immune response, and inflammation. Here, a functional selection strategy was used to isolate novel phagocytosis-promoting genes. After the retroviral transfer of mouse brain cDNA ... More

Quantitation of protein.

Authors:Noble JE, Bailey MJ,

Journal:Methods Enzymol

PubMed ID:19892168

The measurement of protein concentration in an aqueous sample is an important assay in biochemistry research and development labs for applications ranging from enzymatic studies to providing data for biopharmaceutical lot release. Spectrophotometric protein quantitation assays are methods that use UV and visible spectroscopy to rapidly determine the concentration of ... More

A comparison of protein quantitation assays for biopharmaceutical applications.

Authors:Noble JE, Knight AE, Reason AJ, Di Matola A, Bailey MJ,

Journal:Mol Biotechnol

PubMed ID:17914170

Dye-based protein determination assays are widely used to estimate protein concentration, however various reports suggest that the response is dependent on the composition and sequence of the protein, limiting confidence in the resulting concentration estimates. In this study a diverse set of model proteins representing various sizes of protein and ... More

Targeted quantitation of overexpressed and endogenous cystic fibrosis transmembrane conductance regulator using multiple reaction monitoring tandem mass spectrometry and oxygen stable isotope dilution.

Authors:Jiang H, Ramos AA, Yao X,

Journal:Anal Chem

PubMed ID:19947594

Cystic fibrosis transmembrane conductance regulator (CFTR) functions as an ion channel in the apical plasma membrane of epithelial cells. Mutations in the gene coding for CFTR cause cystic fibrosis (CF). A major cellular dysfunction is insufficient apical plasma membrane expression of the protein. Its correction is important for developing new ... More

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