Qubit NGS Library Quantification Assay Kit

The Invitrogen™ Qubit™ NGS Library Quantification Assay is an easy-to-use fluorescence-based assay for precise molar quantification of sequencing-ready Illumina next-generation sequencing (NGS) libraries using the Qubit™ Flex Fluorometer or compatible fluorescence microplate readers. The assay selectively quantifies P5/P7-adapted double-stranded DNA libraries prior to sequencing, helping ensure optimal cluster density and successful sequencing runs.

Accurate determination of library concentration is a critical step in NGS workflows, as the amount of DNA loaded onto a flow cell directly impacts cluster density, data quality, and sequencing efficiency. The Qubit NGS Library Quantification Assay provides a dilution-free, time-saving alternative to qPCR-based library quantification methods while delivering comparable precision.

Key features and benefits:

• Selective molar quantification—measures only dual-adapted, sequencing-ready libraries for improved accuracy
• Dilution-free workflow—eliminates error-prone serial dilution steps required for qPCR
• Broad dynamic range—quantifies libraries from 0.8–46 nM without additional dilution
• Robust performance—accurately quantifies target libraries even in the presence of contaminating nucleic acids
• Flexible detection—compatible with the Qubit Flex Fluorometer and fluorescence microplate readers
• Accessible and cost-effective—no need for dedicated qPCR instrumentation

The streamlined workflow reduces hands-on time and minimizes potential user error, enabling efficient library quantification for both genomic core facilities and individual laboratories. By delivering precise quantification of sequencing-ready libraries, the Qubit NGS Library Quantification Assay helps ensure optimal cluster density on flow cells, improving sequencing outcomes.

Notes

• Designed for use with the Qubit Flex Fluorometer (Cat. No. Q33327) and fluorescence-based microplate readers. The assay is not currently available for use on Qubit 4 Fluorometers.
• Optimized for use with Qubit Flex Pyrogen Free Assay Tube Strips (Cat. No. Q32893).
• Requires the use of the Qubit Flex System Verification Assay Kit (Cat. No. Q33254) prior to the first assay run.