Rhod-2, AM, cell permeant
Rhod-2, AM, cell permeant
Invitrogen™

Rhod-2, AM, cell permeant

표지된 칼슘 지표(calcium indicator)는 Ca2+ 결합 시 형광이 증강되는 분자입니다. 이들은 자가형광 수준이 높은 세포 및 조직에서 Ca2+를 측정하거나 광수용체자세히 알아보기
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카탈로그 번호수량
R12441 mg
R1245MP20 x 50 μg
카탈로그 번호 R1244
제품 가격(KRW)
782,000
Online offer
Ends: 31-Mar-2026
893,000
할인액 111,000 (12%)
Each
카트에 추가하기
수량:
1 mg
제품 가격(KRW)
782,000
Online offer
Ends: 31-Mar-2026
893,000
할인액 111,000 (12%)
Each
카트에 추가하기
표지된 칼슘 지표(calcium indicator)는 Ca2+ 결합 시 형광이 증강되는 분자입니다. 이들은 자가형광 수준이 높은 세포 및 조직에서 Ca2+를 측정하거나 광수용체 및 광활성 킬레이터로 인한 Ca2+ 분비를 검출하는 등 수 많은 칼슘 신호전달 연구에 사용됩니다. 용해된 표지자를 세포 배양 접시에 직접 첨가하여 이런 칼슘 지표의 AM ester 형을 세포에 loading할 수 있습니다. 이런 세포에서 나오는 형광 신호는 대개 fluorescence microscopy로 측정됩니다.

Calcium Indicator (AM Ester) 사양:
• Label (Ex/Em Ca2+–bound form): Rhod-2 (552/581 nm)
• Ca2+ 결합 시 형광 강도가 >100배 증강.
• buffer에 Mg2+가 없는 상태에서 Ca2+의 Kd: ∼570 μM
• 파장 변화가 거의 없이 Ca2+ 결합 시 형광 증강


TPEN을 사용해 중금속 양이온 제어
또한, 이와 같은 BAPTA 기반 표지자는 다양한 중금속 양이온(예: Mn2+, Zn2+, Pb2+)에 Ca2+ 보다 높은 친화성으로 결합함. 중금속 선택적 킬레이터 TPEN를 사용해 이런 이온의 유무로 유발되는 칼슘 측정 변화를 제어할 수 있습니다.

그 밖의 형광 칼슘 표지자
본사는 누출 감소 및 구획화를 위한 dextran 버전, high-amplitude calcium transients 검출을 위한 BAPTA conjugate 등 다양한 실험에 사용할 수 있는 폭넓은 Molecular Probes™ 칼슘 지표를 제공합니다. 더 많은 정보는 Molecular Probes™ Handbook에서 가시광선으로 여기되는 형광 Ca2+ 표지자—Section 19.3를 참조하십시오..

UV로 여기가능한 Ca2+ 지표, 단백질 기반 Ca2+ 지표, Ca2+ 지표 conjugate 및 다른 금속 이온(i.e., Mg2+, Zn2+)의 형광 기반 지표에서는 Molecular Probes™ Handbook에서 Ca2+, Mg2+, Zn2+ 및 기타 금속 이온 지표—Chapter 19를 참조하십시오.

연구용으로만 사용할 수 있습니다. 사람이나 동물의 치료 또는 진단용으로 사용할 수 없습니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
염료 유형Fluorescent Dye-Based
수량1 mg
배송 조건Room Temperature
용도(애플리케이션)Cell Viability and Proliferation
용도 (장비)Fluorescence Microscope
제품 유형Stain
Unit SizeEach
구성 및 보관
Store in freezer -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

I am doing calcium flux imaging with your Fura-2 calibration kit, but am seeing a large variability in ratio in different places around the slide. I am correcting for uniform illumination, using the product as directed, and sealing the coverslip with nail polish.

The nail polish may be the problem. The Kd value (calcium sensitivity) changes depending upon the dye's environment. Nail polish has solvents that can leech under the coverslip and cause variability. We recommend either going without a sealing or sealing with melted paraffin painted on the coverslip edges with a cotton-tipped applicator (paraffin is hydrophobic and has no solvents).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to label cells with Fluo-4, AM, for a calcium flux assay. How long after labeling will the dye be retained?

After loading dye into the cells, intracellular esterases remove the 'AM' moiety from the dye. When the 'AM' group is removed, the dye is able to bind calcium and fluoresce. Since the dye is not covalently bound to any cellular components, it may be actively effluxed from the cell. The rate of efflux is dependent upon the inherent properties of the cell, culture conditions and other factors. The dye may be retained for hours, days or even weeks or lost in a matter of minutes. The use of Probenecid (Cat. No. P36400) limits loss by active efflux.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the excitation/emission maxima for Rhod-2, AM, cell permeant (Cat. No. R1244, R1245MP)?

Rhod-2, AM, cell permeant (Cat. No. R1244, R1245MP) exhibits >100-fold increase in fluorescence intensity upon binding Ca2+. The excitation/emission maxima for Rhod-2, AM, cell permeant when bound to Ca2+ are 552/581 nm.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (140)

인용 및 참조 문헌
Abstract
Functional implications of calcium permeability of the channel formed by pannexin 1.
Authors:Vanden Abeele F,Bidaux G,Gordienko D,Beck B,Panchin YV,Baranova AV,Ivanov DV,Skryma R,Prevarskaya N
Journal:The Journal of cell biology
PubMed ID:16908669
Although human pannexins (PanX) are homologous to gap junction molecules, their physiological function in vertebrates remains poorly understood. Our results demonstrate that overexpression of PanX1 results in the formation of Ca(2+)-permeable gap junction channels between adjacent cells, thus, allowing direct intercellular Ca(2+) diffusion and facilitating intercellular Ca(2+) wave propagation. More ... More
Spatially organised mitochondrial calcium uptake through a novel pathway in chick neurones.
Authors:Coatesworth W, Bolsover S
Journal:Cell Calcium
PubMed ID:16338004
'A brief depolarisation of chick sensory neurones evokes a calcium increase in mitochondria that peaks 1-2s after the depolarisation event and then decays over tens of seconds. Peripheral mitochondria take up more calcium than do central ones, even when the cytosolic calcium increase is spatially homogeneous. The calcium influx into ... More
Feedback inhibition of sodium/calcium exchange by mitochondrial calcium accumulation.
Authors:Opuni K, Reeves JP
Journal:J Biol Chem
PubMed ID:10801871
'Chinese hamster ovary cells expressing the bovine cardiac Na(+)/Ca(2+) exchanger were subjected to two periods of 5 and 3 min, respectively, during which the extracellular Na(+) concentration ([Na(+)](o)) was reduced to 20 mm; these intervals were separated by a 5-min recovery period at 140 mm Na(+)(o). The cytosolic Ca(2+) concentration ... More
Hydrolysis of Ca2+-sensitive fluorescent probes by perfused rat heart.
Authors:Scaduto RC, Grotyohann LW
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:14561682
'Rat hearts were loaded with the fluorescent calcium indicators fura 2, indo 1, rhod 2, or fluo 3 to determine cytosolic calcium levels in the perfused rat heart. With fura 2, however, basal tissue fluorescence increased above anticipated levels, suggesting accumulation of intermediates of fura 2-AM deesterification. To examine this ... More
Nitric oxide-dependent mitochondrial biogenesis generates Ca2+ signaling profile of lupus T cells.
Authors:Nagy G, Barcza M, Gonchoroff N, Phillips PE, Perl A
Journal:J Immunol
PubMed ID:15356113
'Abnormal T cell activation and cell death underlie the pathology of systemic lupus erythematosus. Although mitochondrial hyperpolarization (MHP) represents an early and reversible checkpoint of T cell activation and apoptosis, lupus T cells exhibit persistent MHP. NO has recently been recognized as a key signal of mitochondrial biogenesis and mediator ... More