Blasticidin S HCl, powder
Product Image
인용 및 참조 문헌 (24)
Gibco™

Blasticidin S HCl, powder

Blasticidin S는 Streptomyces griseochromogenes에서 분리한 뉴클레오시드 항생제입니다. 이는 원핵세포와 진핵세포 모두에서 단백질 합성을 억제합니다. Blasticidin S는 ribosome으로 펩티드 결합 형성을자세히 알아보기
Have Questions?
카탈로그 번호수량
R2100150 mg
카탈로그 번호 R21001
제품 가격(KRW)
448,000
Online offer
Ends: 31-Dec-2025
471,000
할인액 23,000 (5%)
Each
카트에 추가하기
수량:
50 mg
제품 가격(KRW)
448,000
Online offer
Ends: 31-Dec-2025
471,000
할인액 23,000 (5%)
Each
카트에 추가하기
Blasticidin S는 Streptomyces griseochromogenes에서 분리한 뉴클레오시드 항생제입니다. 이는 원핵세포와 진핵세포 모두에서 단백질 합성을 억제합니다. Blasticidin S는 ribosome으로 펩티드 결합 형성을 억제하여 신속하게 작용합니다.. Blasticidin S는 세포 배양의 오염을 방지하기 위해 사용합니다. 권장 작업 농도는 cell line에 따라 2∼ 20 μg ⁄ ml 범위입니다. 이 제품은 20mM HEPES(pH 7.2-7.5)에 든10 mg ⁄ ml 용액으로 제공됩니다. 라이프 테크놀로지스는 세포 배양 어플리케이션에 사용하는 다양한 항생∙항진균제 를 제공합니다.

또한 Blasticidin S는 50 - 100 μg ⁄ ml 농도에서 박테리아 선택적인 항생제로 사용됩니다. 내성은 Blasticidin S deaminase 유전자 BSD (2) 또는 bsr 중 하나의 발현으로 확인합니다.이 deaminases가 Blasticidin S를 무독성 변이체로 바꿉니다. 선택 용으로는 LB medium의 염함유량은 낮고(<90 mM) pH는 7.0를 초과하지 않아야 Basticidin S 활성을 유지할 수 있습니다. 라이프테크놀로지스는 다양한선택 항생제를 제공합니다.

용도

이 제품은 연구용으로만 사용해야 합니다. 치료 또는 진단 목적으로 동물이나 인간에 사용할 수 없습니다.

cGMP 제조 품질 시스템
Gibco™ Blasticidin S HCl은 뉴욕 Grand Island에 소재한 cGMP에 부합하는 시설에서 제조됩니다. 이 시설은 의료기기 제조자로 FDA에 등록되어 있으며 ISO 13485, ISO 9001 인증을 받은 기관입니다.
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형Eukaryotic Cells, Prokaryotic Cells
색상White, off-white
농도50 to 100 μg/mL
배양 유형Mammalian Cell Culture, Insect Cell Culture
용도(애플리케이션)Bacterial Selection
제품라인Gibco
수량50 mg
유통 기한Lot Dependent
형태Powder
제품 유형Antibiotic
멸균Sterile
첨가제 포함HEPES
Unit SizeEach
구성 및 보관
Storage conditions: -5 to -30°C
Shipping conditions: room temperature
Shelf life: Lot Dependent

자주 묻는 질문(FAQ)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

View all Blasticidin S HCl, powder FAQs

인용 및 참조 문헌 (24)

인용 및 참조 문헌
Abstract
Suppression of Ras-mediated tumorigenicity and metastasis through inhibition of the Met receptor tyrosine kinase.
Authors: Furge K A; Kiewlich D; Le P; Vo M N; Faure M; Howlett A R; Lipson K E; Woude G F; Webb C P;
Journal:Proc Natl Acad Sci U S A
PubMed ID:11535809
'Mutations in the Ras family of GTP binding proteins represent one of the most frequently observed genetic alterations in human cancers. We and others have recently demonstrated that expression of Met, the tyrosine kinase receptor for hepatocyte growth factor/scatter factor (HGF/SF), is significantly up-regulated in Ras-transformed cells. Because HGF/SF-Met signaling ... More
Structural basis of G protein specificity of human endothelin receptors. A study with endothelinA/B chimeras.
Authors: Takagi Y; Ninomiya H; Sakamoto A; Miwa S; Masaki T;
Journal:J Biol Chem
PubMed ID:7730310
'The endothelin (ET) family of peptides acts via two subtypes of guanine nucleotide-binding regulatory protein (G protein)-coupled receptors termed ETA and ETB. ET-1 stimulated cAMP formation in Chinese hamster ovary (CHO) cells stably expressing human wild-type ETA (CHO/hETA cells) while it inhibited cAMP formation in CHO cells expressing human wild-type ... More
West Nile virus discriminates between DC-SIGN and DC-SIGNR for cellular attachment and infection.
Authors:Davis CW, Nguyen HY, Hanna SL, Sánchez MD, Doms RW, Pierson TC,
Journal:J Virol
PubMed ID:16415006
'The C-type lectins DC-SIGN and DC-SIGNR bind mannose-rich glycans with high affinity. In vitro, cells expressing these attachment factors efficiently capture, and are infected by, a diverse array of appropriately glycosylated pathogens, including dengue virus. In this study, we investigated whether these lectins could enhance cellular infection by West Nile ... More
Presenilin 1 mutations activate gamma 42-secretase but reciprocally inhibit epsilon-secretase cleavage of amyloid precursor protein (APP) and S3-cleavage of notch.
Authors: Chen Fusheng; Gu YongJun; Hasegawa Hiroshi; Ruan Xueying; Arawaka Shigeki; Fraser Paul; Westaway David; Mount Howard; St George-Hyslop Peter;
Journal:J Biol Chem
PubMed ID:12119298
'The presenilin 1 (PS1) and presenilin 2 (PS2) proteins are necessary for proteolytic cleavage of the amyloid precursor protein (APP) within its transmembrane domain. One of these cleavage events (termed gamma-secretase) generates the C-terminal end of the Abeta-peptide by proteolysis near residue 710 or 712 of APP(770). Another event (termed ... More
Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic beta-cell line.
Authors:Thomas H, Senkel S, Erdmann S, Arndt T, Turan G, Klein-Hitpass L, Ryffel GU,
Journal:Nucleic Acids Res
PubMed ID:15520459
'Using the rat insulinoma cell line INS-1 we generated beta-cell clones that are most efficient for gene transfer, as they contain an FRT site for Flp recombinase-mediated, site-directed integration of a single copy transgene. Therefore, the gene-of-interest can be introduced by DNA transfection without the need to select individual cell ... More
View all Blasticidin S HCl, powder citations