TO-PRO™3 Ready Flow™ Reagent
TO-PRO™3 Ready Flow™ Reagent
인용 및 참조 문헌 (1)
Invitrogen™

TO-PRO™3 Ready Flow™ Reagent

TO-PRO™3 Ready Flow™ Reagent is a bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells in자세히 알아보기
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카탈로그 번호수량
R37170120 assays
카탈로그 번호 R37170
제품 가격(KRW)
493,000
キャンペーン価格
Ends: 31-Dec-2025
580,000
할인액 87,000 (15%)
Each
카트에 추가하기
수량:
120 assays
제품 가격(KRW)
493,000
キャンペーン価格
Ends: 31-Dec-2025
580,000
할인액 87,000 (15%)
Each
카트에 추가하기
TO-PRO™3 Ready Flow™ Reagent is a bright and easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. This dye has excitation/emission maxima of 642/661 nm, similar to Alexa Fluor™ 647 or allophycocyanin (APC).

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Rapid staining of dead cells without wash steps

TO-PRO 3 Ready Flow Reagent is a ready-to-use, direct-to-sample dead-cell stain that emits red fluorescence when bound to DNA. Add 2 drops form the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

TO-PRO 3 Ready Flow Reagent is membrane impermeant and therefore excluded from viable cells. It binds selectively to double-stranded DNA. This dye has excitation/emission maxima of 642/661 nm.

사양
세포 투과성Impermeant
설명TO-PRO™3 Ready Flow™ Reagent
검출 방법Fluorescence
염료 유형Cell-Impermeant
형태Solution
형식Bottle(s)
수량120 assays
용해도DMSO (Dimethylsulfoxide)
세포하위구조 국지화Nucleus
색상Far-red
Emission661
Excitation Wavelength Range642 nm
용도(애플리케이션)Viability Assay
용도 (장비)Flow Cytometer
제품라인Ready Flow, TO-PRO3
제품 유형Reagent
Unit SizeEach
구성 및 보관
Includes 2 dropper bottles, store at room temperature.

자주 묻는 질문(FAQ)

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Pigment epithelium-derived factor hinders photoreceptor cell death by reducing intracellular calcium in the degenerating retina.
Authors:Comitato A, Subramanian P, Turchiano G, Montanari M, Becerra SP, Marigo V,
Journal:Cell Death Dis
PubMed ID:29752430
Calcium ions play a critical role in neuronal cell death. Pigment epithelium-derived factor (PEDF) is a promising neuroprotective protein for photoreceptor cells but the mechanisms mediating its effects against retinal degeneration are still not well characterized. We addressed this question in the rd1 degenerating mouse retina that bears a mutation ... More