SYTO™ 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

인용 및 참조 문헌 (25)

Invitrogen™

SYTO™ 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

세포 침투성 SYTO 60 red fluorescent nucleic acid stain은 핵산과 결합하여 밝은 적색 형광을 띱니다. 살아있는 세포에서 SYTO dye의 염색자세히 알아보기

카탈로그 번호	수량
S11342	250 μL

카탈로그 번호 S11342

제품 가격(KRW)

431,000

キャンペーン価格

Ends: 31-Dec-2025

507,000

할인액 76,000 (15%)

카트에 추가하기

250 μL

제품 가격(KRW)

431,000

キャンペーン価格

Ends: 31-Dec-2025

507,000

할인액 76,000 (15%)

카트에 추가하기

세포 침투성 SYTO 60 red fluorescent nucleic acid stain은 핵산과 결합하여 밝은 적색 형광을 띱니다. 살아있는 세포에서 SYTO dye의 염색 패턴은 세포유형마다 다를 수 있기 때문에 연구자들이 각자 시스템에 가장 적합한 적색 형광 SYTO 염색을 찾을 수 있도록 SYTO Red Fluorescent Nucleic Acid Stain Sampler Kit (S-11340)를 제공합니다.

For Research Use Only. Not for use in diagnostic procedures.

색상Red

설명SYTO™ 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

검출 방법Fluorescence

염료 유형Cell-Permeant

방출678 nm

여기 파장 범위652 nm

용도(장비)Fluorescence Microscope

제품라인SYTO

수량250 μL

배송 조건Room Temperature

부피(미터법)250 μL

라벨 유형Fluorescent Dye

제품 유형Nucleic Acid Stain

SubCellular LocalizationNucleic Acids

Unit SizeEach

구성 및 보관

Store in freezer at -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (25)

인용 및 참조 문헌

Abstract

The protein tyrosine phosphatase PTP-BL associates with the midbody and is involved in the regulation of cytokinesis.

Authors:Herrmann L, Dittmar T, Erdmann KS

Journal:Mol Biol Cell

PubMed ID:12529439

'PTP-BL is a highly modular protein tyrosine phosphatase of unknown function. It consists of an N-terminal FERM domain, five PDZ domains, and a C-terminally located tyrosine phosphatase domain. Here we show that PTP-BL is involved in the regulation of cytokinesis. We demonstrate localization of endogenous PTP-BL at the centrosomes during ... More

Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.

Authors:Pin S, Chen H, Lein PJ, Wang MM

Journal:J Neurochem

PubMed ID:16441515

'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More

Multiparameter detection of apoptosis using red-excitable SYTO probes.

Authors:Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z,

Journal:Cytometry A

PubMed ID:18431792

'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More

Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.

Authors:Huang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F

Journal:Biochemistry

PubMed ID:14992586

'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More

Cytoplasmic and nuclear delivery of a TAT-derived peptide and a beta-peptide after endocytic uptake into HeLa cells.

Authors:Potocky TB, Menon AK, Gellman SH

Journal:J Biol Chem

PubMed ID:14517218

'Several short, highly cationic peptides are able to enter the cytoplasm and nucleus of cells from the extracellular medium. The mechanism of entry is unknown. A number of fluorescence-based studies suggested that these molecules cross the plasma membrane by an energy-independent process, directly gaining access to the cytoplasm. Recent reports ... More

View all SYTO™ 60 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO citations