SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

인용 및 참조 문헌 (8)

Invitrogen™

SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

The cell-permeant SYTO 41 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the자세히 알아보기

카탈로그 번호	수량
S11352	250 μL

카탈로그 번호 S11352

제품 가격(KRW)

250 μL

The cell-permeant SYTO 41 blue fluorescent nucleic acid stain exhibits bright, blue fluorescence upon binding to nucleic acids. Because the staining pattern of the SYTO dyes in live cells may vary from one cell type to another, we offer the SYTO Blue Fluorescent Nucleic Acid Stain Sampler Kit (Cat. No. S-11350) to enable researchers to find the dye that works best for a particular application.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

For Research Use Only. Not for use in diagnostic procedures.

색상Blue

설명SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

검출 방법Fluorescence

염료 유형Cell-Permeant

방출454 nm

여기 파장 범위430 nm

용도(장비)Fluorescence Microscope

제품라인SYTO

수량250 μL

배송 조건Room Temperature

부피(미터법)250 μL

라벨 유형Fluorescent Dye

제품 유형Nucleic Acid Stain

SubCellular LocalizationNucleic Acids

Unit SizeEach

구성 및 보관

Store in freezer at -5°C to -30°C and protect from light.

자주 묻는 질문(FAQ)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (8)

인용 및 참조 문헌

Abstract

Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.

Authors:Pin S, Chen H, Lein PJ, Wang MM

Journal:J Neurochem

PubMed ID:16441515

'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More

Imaging collagen in intact viable healthy and atherosclerotic arteries using fluorescently labeled CNA35 and two-photon laser scanning microscopy.

Authors:Megens RT, Oude Egbrink MG, Cleutjens JP, Kuijpers MJ, Schiffers PH, Merkx M, Slaaf DW, van Zandvoort MA,

Journal:Mol Imaging

PubMed ID:17711780

We evaluated CNA35 as a collagen marker in healthy and atherosclerotic arteries of mice after both ex vivo and in vivo administration and as a molecular imaging agent for the detection of atherosclerosis. CNA35 conjugated with fluorescent Oregon Green 488 (CNA35/OG488) was administered ex vivo to mounted viable muscular (uterine), ... More

Two pathways converge at CED-10 to mediate actin rearrangement and corpse removal in C. elegans.

Authors:Kinchen JM, Cabello J, Klingele D, Wong K, Feichtinger R, Schnabel H, Schnabel R, Hengartner MO

Journal:Nature

PubMed ID:15744306

The removal of apoptotic cells is essential for the physiological well being of the organism. In Caenorhabditis elegans, two conserved, partially redundant genetic pathways regulate this process. In the first pathway, the proteins CED-2, CED-5 and CED-12 (mammalian homologues CrkII, Dock180 and ELMO, respectively) function to activate CED-10 (Rac1). In ... More

Two-photon microscopy of vital murine elastic and muscular arteries. Combined structural and functional imaging with subcellular resolution.

Authors:Megens RT, Reitsma S, Schiffers PH, Hilgers RH, De Mey JG, Slaaf DW, oude Egbrink MG, van Zandvoort MA

Journal:J Vasc Res

PubMed ID:17192719

Understanding vascular pathologies requires insight in the structure and function, and, hence, an imaging technique combining subcellular resolution, large penetration depth, and optical sectioning. We evaluated the applicability of two-photon laser-scanning microscopy (TPLSM) in large elastic and small muscular arteries under physiological conditions. Elastic (carotid) and muscular (uterine, mesenteric) arteries ... More

Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis.

Authors:

Journal:Cell Host Microbe

PubMed ID:33497603

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