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Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)
인용 및 참조 문헌 (1)
Invitrogen™

Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)

Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)
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카탈로그 번호수량
S21008
S-21008으로도 사용됨
100 μL
카탈로그 번호 S21008
S-21008으로도 사용됨
제품 가격(KRW)
599,000
Each
수량:
100 μL
제품 가격(KRW)
599,000
Each

The streptavidin, Alexa Fluor™ 750-allophycocyanin (APC) streptavidin tandem conjugate has excitation and emission maxima at ∼650/779 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications following excitation at 633 nm or 647 nm.

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For Research Use Only. Not for use in diagnostic procedures.

사양
농도1 mg/mL
검출 방법Fluorescence
여기/방출633 or 635/775
유세포분석기 레이저 라인633 or 635
용도(장비)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
라벨 또는 염료APC-Alexa Fluor 750
제품 유형Streptavidin Conjugate (Fluorescent)
수량100 μL
배송 조건Wet Ice
콘주게이트APC-Alexa Fluor 750
형태Solution
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
Contains 1 vial of streptavidin APC-Alexa Fluor™ 750 conjugate (1 mg/mL).

Store in refrigerator (2–8°C) and protect from light.

자주 묻는 질문(FAQ)

Can I use my tandem dye antibody (e.g., Alexa Fluor 610-R-phycoerythrin) for microscopy?

Tandem dyes are not recommended for imaging applications. Both R-PE or APC tend to photobleach quickly and cannot be archived. Any unfolding of the phycobiliprotein would result in loss of fluorescence. They work great in flow cytometry applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store my allophycocyanin (APC)-conjugated antibody?

Allophycocyanin-conjugated antibodies must be stored at 2-8 degrees C. Antibodies conjugated to phycobiliproteins (APC, R-PE) must never be frozen. Once frozen, even briefly, they are no longer usable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates.
Authors:Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP
Journal:J Histochem Cytochem
PubMed ID:14623938
'Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were compared with spectrally similar protein conjugates of the Cy3, Cy5, ... More