Streptavidin, Alexa Fluor™ 555 Conjugate
Streptavidin, Alexa Fluor™ 555 Conjugate
Invitrogen™

Streptavidin, Alexa Fluor™ 555 Conjugate

Alexa Fluor™ 555 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has자세히 알아보기
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카탈로그 번호수량형태
S213811 mgSolid
S323550.5 mLLiquid
카탈로그 번호 S21381
제품 가격(KRW)
393,000
線上優惠
Ends: 31-Dec-2025
462,000
할인액 69,000 (15%)
Each
카트에 추가하기
수량:
1 mg
형태:
Solid
제품 가격(KRW)
393,000
線上優惠
Ends: 31-Dec-2025
462,000
할인액 69,000 (15%)
Each
카트에 추가하기

Alexa Fluor™ 555 streptavidin comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.

Important Features of Alexa Fluor™ 555 Streptavidin Conjugates:
Alexa Fluor™ 555 streptavidin conjugate has Ex/Em maxima of ∼ (555/565)
Bright, photostable fluorescence
High solubility in aqueous solutions
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability and brightness and improved solubility in aqueous solutions. Available in a broad range of colors, these dyes are a good choice for most imaging applications.

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

See User Manual for solubility instructions.

Related Links:
Learn more about Avidin-Biotin Detection
Learn more about Alexa Fluor™ Dyes
Find out about other Labeled Streptavidin Conjugates
Read Avidin and Streptavidin Conjugates-Section 7.6 in the Molecular Probes Handbook

For Research Use Only. Not for use in diagnostic procedures.
사양
여기/방출555/565 nm
라벨 또는 염료Alexa Fluor
제품 유형Streptavidin Conjugate (fluorescent)
수량1 mg
배송 조건Room Temperature
콘주게이트Alexa Fluor 555
형태Solid
제품라인Alexa Fluor
Unit SizeEach
구성 및 보관
Store in freezer (-5°C to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

인용 및 참조 문헌 (10)

인용 및 참조 문헌
Abstract
Seeing the trees in the forest: selective electroporation of adipocytes within adipose tissue.
Authors:Granneman JG, Li P, Lu Y, Tilak J
Journal:Am J Physiol Endocrinol Metab
PubMed ID:15126244
'Electroporation has been recently adapted for the transfer of macromolecules into cells of tissues in vivo. Although mature adipocytes constitute <20% of cells residing in adipose tissue, we hypothesized that fat cells might be susceptible to selective electrotransfer of plasmid DNA owing to their large size relative to other cells ... More
PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.
Authors:Golestaneh N, Fan J, Zelenka P, Chepelinsky AB,
Journal:Mol Vis
PubMed ID:18523655
'PURPOSE: To investigate the functional significance of MIP/AQP0 phosphorylation at serine(235). METHODS: MIP/AQP0 expression and cellular localization was studied in rat lens epithelia explants induced to differentiate by FGF-2. MIP wild type (WT) and MIP (S235A) mutant expression plasmids were constructed and transiently expressed in RK13 cells. Subcellular localization of ... More
Collagen XVI harbors an integrin alpha1 beta1 recognition site in its C-terminal domains.
Authors:Eble JA, Kassner A, Niland S, Mörgelin M, Grifka J, Grässel S
Journal:J Biol Chem
PubMed ID:16754661
'Collagen XVI is integrated tissue-dependently into distinct fibrillar aggregates, such as D-banded cartilage fibrils and fibrillin-1-containing microfibrils. In skin, the distribution of collagen XVI overlaps that of the collagen-binding integrins alpha1 beta1 and alpha2 beta1. Basal layer keratinocytes express integrin alpha2 beta1, whereas integrin alpha1 beta1 occurs in smooth muscle ... More
Diabetes is predicted by the beta cell level of autoantigen.
Authors:Byersdorfer CA, Schweitzer GG, Unanue ER
Journal:J Immunol
PubMed ID:16177075
'Two novel transgenic (Tg) strains were created expressing hen egg-white lysozyme (HEL) in a pancreas-specific fashion. RmHP.111 mice had levels of HEL per cell similar to that of the established ILK-3 strain, while RmHP.117 mice had 10-fold lower levels (50,000 molecules per cell). When bred to 3A9 TCR Tg mice, ... More
Metabolic and cellular plasticity in white adipose tissue II: role of peroxisome proliferator-activated receptor-alpha.
Authors:Li P, Zhu Z, Lu Y, Granneman JG
Journal:Am J Physiol Endocrinol Metab
PubMed ID:15941786
'Chronic activation of adipocyte beta-adrenergic receptors induces remodeling of white adipose tissue (WAT) that includes a transient inflammatory response followed by mitochondrial biogenesis, induction of fatty acid oxidation genes, and elevation of tissue oxidative metabolism. Gene profiling experiments of WAT during remodeling induced by the beta(3)-adrenergic receptor agonist CL-316,243 (CL) ... More