Streptavidin, Pacific Orange™ conjugate

인용 및 참조 문헌 (2)

Invitrogen™

Streptavidin, Pacific Orange™ conjugate

Pacific Orange™ streptavidin has absorption/emission maxima of ∼400/551 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria자세히 알아보기

카탈로그 번호	수량
S32365	1 mg

카탈로그 번호 S32365

제품 가격(KRW)

475,000

카트에 추가하기

제품 가격(KRW)

475,000

카트에 추가하기

Pacific Orange™ streptavidin has absorption/emission maxima of ∼400/551 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

For Research Use Only. Not for use in diagnostic procedures.

검출 방법Fluorescence

여기/방출400/551

유세포분석기 레이저 라인405

용도(장비)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis

라벨 또는 염료Pacific Orange

제품 유형Streptavidin Conjugate (Fluorescent)

수량1 mg

배송 조건Room Temperature

콘주게이트Pacific Orange

형태Solid

제품라인Pacific Orange

Unit SizeEach

구성 및 보관

Contains 1 vial of streptavidin Pacific Orange™ conjugate.

Store in freezer (-5 to -30°C) and protect from light.

자주 묻는 질문(FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

인용 및 참조 문헌 (2)

인용 및 참조 문헌

Abstract

An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform.

Authors:Wang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA,

Journal:J Immunol Methods

PubMed ID:19049809

Human peripheral blood dendritic cells (PBDC) are a rare population comprised of several distinctive subsets. Analysis of these cells has been hindered by their low frequency. In this study, we report a novel direct ex vivo 11-color flow cytometric assay that combines subset identification with analysis of activation status and ... More

Violet laser diodes in flow cytometry: an update.

Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,

Journal:Cytometry A

PubMed ID:17051581

INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More