Invitrogen™

SYTO™ RNASelect™ Red

Name: SYTO™ RNASelect™ Red
SKU: S32710

보기 방식 변경

카탈로그 번호	수량
S32710	300 μL
S32711	3 x 300 μL

2 개 옵션

카탈로그 번호 S32710

제품 가격(KRW)

수량:

300 μL

SYTO™ RNASelect™ Red is a cell-permeant, RNA-selective fluorescent dye for live or fixed eukaryotic cells. It allows researchers to visualize RNA-rich nucleoli and cytoplasmic RNA in a Texas Red/RFP compatible channel. Compared to SYTO™ RNASelect™ Green, it is compatible with 4% paraformaldehyde fixation rather than methanol fixation, and leaves GFP/FITC channels open for antibodies, fluorescent proteins, and other fluorescent probes in multiplex and high-content imaging.

This molecular probe is prepared as a ready-to-use 100x aqueous solution, appropriate for direct addition to either complete media or other buffers of your choice to then be used in staining cells.

Compatibility with 4% paraformaldehyde fixation and permeabilization expands the applicability of this RNA dye for immunofluorescence workflows, enabling its use in high content screening for nucleolar reorganization, cell stress assays, and deep learning computational models.

The staining patterns demonstrate high RNA specificity, with expected staining demonstrating bright nucleoli and diffuse ribosomal staining throughout the cytoplasm and is easily multiplexable with classic nuclear stains such as Hoechst 33342 or DAPI. This specificity is demonstrated by the sharp decrease in staining intensity upon treatment with RNase, all red signal is quickly depleted while the nuclear DNA dye staining remains intact.

Features

Suitable for both live and fixed cell workflows, connecting live cell observation with endpoint imaging.
Compatible with multiplex imaging outside of the Texas Red/RFP channels with various organelle markers such as Hoechst/DAPI, phalloidin, ER, lectins, or mitochondrial probes.
Applicable to high-content imaging and RNA based nucleolar morphology studies, including workflows where nucleolar signal is used for segmentation or comparative phenotyping.

For researchers familiar with SYTO™ 14, SYTO™ RNASelect™ Red offers a red channel option for RNA associated morphology in Cell Painting related or other multiplex imaging workflows.

This dye complements SYTO™ RNASelect™ Green in channel planning and fixation choice: SYTO™ RNASelect™ Green remains the better fit when the green channel is desired and the sample type requires methanol fixation, whereas SYTO™ RNASelect™ Red is appropriate when a red RNA channel or 4% PFA workflow is preferred.

Available as a single vial as S32710 and in a 3-pack format as S32711.

For Research Use Only.

사양

세포 투과성Live and Fixed Cell Permeable

색상Red

농도1 mM in 25% DMSO/DI H2O

검출 방법Fluorescence with Texas Red Filter Set

염료 유형Fluorogenic Organic Dye

방출626 nm

여기 파장 범위595 nm

용도(애플리케이션)Live Cell Imaging, Fixed Cell Imaging, High Content Screening, Fluorescence Microscopy

용도(장비)Fluorescence Microscope, Spectral Imaging Instrument, Flow Cytometer, High Content Imager

형태Aqueous Liquid with 25% DMSO

형식Vial

테스트 수300 Tests

제품라인SYTO RNASelect

수량300 μL

반응성RNA, Nucleolar Structure

시약 유형Fluorogenic RNA Label

타겟RNA

기술High Content Imaging, Fluorescence Microscopy

검증된 애플리케이션Live Cell Imaging, Fixed Cell Imaging, High Content Imaging

부피(미터법)300 μL

라벨 유형Fluorescent Dye

제품 유형RNA-specific Label

Sub Cellular LocalizationNucleolus

Unit SizeEach

구성 및 보관

•1 Vial
•Store at 2°C to 30°C
•Protect from light
•Do not freeze

자주 묻는 질문(FAQ)

How can I prepare my exosome sample (isolated using the Total Exosome Isolation reagent) for analysis by mass spectroscopy?

The Total Exosome Isolation reagent allows recovery of the entire exosome population from the sample, in contrast to ultracentrifugation protocols, which recover significantly less material. Thus, the pellet is larger. The purity of exosomes is very similar for cell media samples (reagent vs ultra); for more challenging samples such as serum (which are more viscous and have much higher protein content, etc.) the reagent recovers exosomes at a lower purity (few microvesicles and large protein complexes co-precipitate) compared to ultracentrifugation. Having said this, you can obtain enough material for all standard types of downstream analysis, such as qRT-PCR or Western blot with either method.

Can I use PBS with SYTO RNASelect Red, SYTO RNASelect Green, and SYTO 14 reagents?

PBS can be acceptable in some workflows, but we do not recommend assuming it is always the best-performing buffer for all three probes. Some applications may perform better in phosphate-free buffers, so we suggest optimizing as needed. When a starting point is needed, we recommend Live Cell Imaging Solution (LCIS; Cat. No. A59688DJ).

Can I use SYTO RNASelect Red, SYTO RNASelect Green, or SYTO 14 reagents as absolute measurements of total RNA content?

We recommend using these stains as relative imaging reagents within a controlled workflow rather than as absolute measurements of total RNA content. SYTO RNASelect Green reagent has been used in quantitative RNA content assay workflows, but any quantitative application should be carefully calibrated and ideally confirmed with an orthogonal RNA quantitation method.

Are SYTO RNASelect Red, SYTO RNASelect Green, and SYTO 14 reagents transcript-specific?

No. We recommend thinking of SYTO RNASelect Red, SYTO RNASelect Green, and SYTO 14 reagent as RNA-associated stains rather than sequence-targeted probes.

Can I use SYTO RNASelect Red, SYTO RNASelect Green, or SYTO 14 reagents for automated segmentation or machine-learning analysis?

Yes. We suggest these dyes can be used for automated segmentation and machine-learning analysis, depending on the workflow. SYTO 14 reagent has strong Cell Painting precedent, and SYTO RNASelect Red reagent has shown utility in high-content imaging workflows that rely on segmentation-oriented nucleolar analysis.

View all SYTO™ RNASelect™ Red FAQs