SYBR™ Safe DNA Gel Stain in 1X TAE
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SYBR™ Safe DNA Gel Stain in 1X TAE
인용 및 참조 문헌 (2)
Invitrogen™

SYBR™ Safe DNA Gel Stain in 1X TAE

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SYBR Safe™ DNA gel stain was developed specifically for reduced mutagenicity—to be safer than ethidium bromide for staining DNA in자세히 알아보기
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카탈로그 번호수량
S331124 L
S331111 L
카탈로그 번호 S33112
제품 가격(KRW)
483,000
온라인 행사
Ends: 31-Dec-2025
536,000
할인액 53,000 (10%)
Each
카트에 추가하기
수량:
4 L
대량 주문 또는 맞춤형 요청
제품 가격(KRW)
483,000
온라인 행사
Ends: 31-Dec-2025
536,000
할인액 53,000 (10%)
Each
카트에 추가하기
SYBR Safe™ DNA gel stain was developed specifically for reduced mutagenicity—to be safer than ethidium bromide for staining DNA in agarose or acrylamide gels. SYBR Safe™stain is not only less mutagenic than ethidium bromide, but SYBR Safe™ stain's detection sensitivity is better than that of ethidium bromide. SYBR Safe™ stain comes as a premixed solution that can be used just like an ethidium bromide solution, either in the gel during the electrophoresis run or as a post-run stain. Bound to nucleic acids, the green-fluorescent SYBR Safe™ stain has fluorescence excitation maxima at ∼280 and ∼502 nm, and an emission maximum at ∼530 nm.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 위치In-Gel Detection
검출 방법Fluorescence
그린 기능Less hazardous
제품 유형DNA Gel Stain
수량4 L
유통 기한6 months from date of purchase
배송 조건Room Temperature
타겟 분자DNA
라벨 또는 염료SYBR Safe
Unit SizeEach
구성 및 보관
• Supplied as a ready-to-use solution in 1X TAE

Store at room temperature in the original container.

자주 묻는 질문(FAQ)

Why do I sometimes see speckles in my gel when using SYBR Safe DNA Gel Stain?

Many whitening agents used in clothing, as well as some fungi and bacteria, fluoresce at the same wavelengths as SYBR Safe DNA gel stain. These contaminants within or on the surface of the gel may produce this speckling.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which direction does the SYBR Safe dye run during electrophoresis?

Similarly to ethidium bromide, SYBR Safe DNA Gel Stain runs in the opposite direction of the migrating DNA. This has no practical effect on the use of gels cast with SYBR Safe DNA Gel Stain, as only the very bottom of the gel will have a lower concentration of stain. This effect can be partially counteracted by staining the gel with SYBR Safe DNA Gel Stain after electrophoresis. Solutions of dye should not be added to the running buffer as this can cause breakdown of the dye at the electrodes and release toxic volatile compounds into the air.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Does ethanol precipitation remove the SYBR Safe dye?

SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I reuse SYBR Safe DNA Gel Stain for a second gel?

We strongly discourage the reuse of SYBR Safe DNA Gel Stain, as this practice significantly lowers sensitivity.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

View all SYBR™ Safe DNA Gel Stain in 1X TAE, 4 L FAQs

인용 및 참조 문헌 (2)

인용 및 참조 문헌
Abstract
Development of a SYBR safe technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays.
Authors:Martineau C, Whyte LG, Greer CW,
Journal:J Microbiol Methods
PubMed ID:18329741
SYBR safe, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of 12C- and 13C-labelled DNA using SYBR safe gave similar results to those obtained using EtBr with pure cultures ... More
Mechanisms of palatal epithelial seam disintegration by transforming growth factor (TGF) beta3.
Authors:Ahmed S, Liu CC, Nawshad A,
Journal:Dev Biol
PubMed ID:17698055
TGFbeta3 signaling initiates and completes sequential phases of cellular differentiation that is required for complete disintegration of the palatal medial edge seam, that progresses between 14 and 17 embryonic days in the murine system, which is necessary in establishing confluence of the palatal stroma. Understanding the cellular mechanism of palatal ... More